Nucleotide sequence analysis of the Pseudomonas putida PpG7 salicylate hydroxylase gene (nahG) and its 3'-flanking region
| Autor: | Debabrota Ghosal, In Soon You, Irwin C. Gunsalus |
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| Rok vydání: | 1991 |
| Předmět: |
Base pair
Sequence analysis Molecular Sequence Data Restriction Mapping Biochemistry Mixed Function Oxygenases chemistry.chemical_compound Open Reading Frames Pseudomonas Sequence Homology Nucleic Acid Escherichia coli Amino Acid Sequence Cloning Molecular Peptide sequence Gene biology Base Sequence Nucleic acid sequence biology.organism_classification Molecular biology Pseudomonas putida Molecular Weight Open reading frame chemistry Genes Bacterial DNA |
| Zdroj: | Biochemistry. 30(6) |
| ISSN: | 0006-2960 |
| Popis: | Gene nahG of naphthalene/salicylate catabolic plasmid NAH7 encodes a protein of molecular weight 45,000, salicylate hydroxylase. This enzyme catalyzes the formation of catechol from salicylate, a key intermediate in naphthalene catabolism. DNA sequence analysis of the 3.1-kilobase HindIII fragment containing the nahG locus reveals an open reading frame (ORF) of 1305 base pairs that corresponds to a protein of 434 amino acid residues. The predicted amino acid sequence of salicylate hydroxylase is in agreement with the molecular weight, NH2-terminal amino acid sequence, and total amino acid composition of the purified salicylate hydroxylase [You, I.-S., Murray, R. I., Jollie, D., & Gunsalus, I. C. (1990) Biochem. Biophys. Res. Commun. 169, 1049-1054]. The amino acid sequence between positions 8 and 37 of salicylate hydroxylase shows homology with known ADP binding sites of other FAD-containing oxidoreductases, thus confirming its biochemical function. The sequence of the Pseudomonas putida salicylate hydroxylase was compared with those of other similar flavoproteins. A small DNA segment (831 base pairs) disrupts the continuity of the known gene order nahG and nahH, the latter encoding catechol 2,3-dioxygenase. The complete nucleotide sequence of the intergenic region spanning genes nahG and nahH has been determined and its biological role proposed. |
| Databáze: | OpenAIRE |
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