Translocating lipopolysaccharide correlates with the severity of enterovirus A71-induced HFMD by promoting pro-inflammation and viral IRES activity

Autor:	Bangtao Chen, Xujiao Feng, Yuya Wang, Kena Dan, Jing Yang, Xiongbo Yang, Xiaoling Xue, Qingqing Yang
Rok vydání:	2021
Předmět:	Lipopolysaccharide CD14 Enterovirus A71 Inflammation RC799-869 Microbiology Internal ribosomal entry site (IRES) chemistry.chemical_compound Western blot Virology medicine 2A protease (2Apro) biology medicine.diagnostic_test business.industry Research Gastroenterology Diseases of the digestive system. Gastroenterology In vitro Internal ribosome entry site Hand foot and mouth disease Infectious Diseases chemistry biology.protein Parasitology Tumor necrosis factor alpha Antibody medicine.symptom business
Zdroj:	Gut Pathogens Gut Pathogens, Vol 13, Iss 1, Pp 1-11 (2021)
ISSN:	1757-4749
Popis:	Background The increase of inflammation-inducing enterobacteria was recently observed in severe hand, foot, and mouth disease (HFMD) caused by Enterovirus A71 (EV-A71). This study aimed to verify the occurrence of bacterial translocation (BT) and further explore the contributory role of BT to severity of EV-A71-mediated HFMD cases. Methods Serum specimens from 65 mild and 65 severe EV-A71-associated HFMD cases and 65 healthy children were collected. EV-A71 VP1 in serum, inflammatory mediators including C-reactive protein, IL-1β, IL-6, interferon-γ and tumor necrosis factor-α, BT related biomarkers including Claudin-3, intestinal fatty acid binding protein, lipopolysaccharide (LPS), soluble CD14 (sCD14) and endotoxin core antibody were measured by ELISA. Bacterial DNA (BactDNA) fragments were quantified by quantified PCR (qPCR). Rhabdomyosarcoma (RD) or SH-SY5Y cells, infected with LPS-pre-incubated EV-A71 or transfected with plasmid containing viral 2Apro or mRNA containing viral internal ribosomal entry site (IRES), were post-treated with or without LPS in vitro. EV-A71 RNA and viral or cellular proteins were determined by qPCR and western blot, respectively. Results Compared to mild HFMD patients, remarkably higher inflammatory mediators as well as BT-related biomarkers except BactDNA were observed in severe HFMD cases (all P P r = 0.341, P = 0.005) and serum sCD14 (r = 0.458, P pro-mediated IRES activity were significantly accelerated by LPS post-treatment. Conclusions Our results collectively indicate that gut-derived translocating LPS contributes to the severity of EV-A71-induced HFMD by driving inflammatory response and viral proliferation via viral 2Apro-mediated IRES.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7f7017e7622d06fd7d2b1247a936eb78 https://pubmed.ncbi.nlm.nih.gov/34809671 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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