Antinociceptive activity of astragaloside IV in the animal model of chronic constriction injury
| Autor: | Qi Wang, Rong Fan, Yu-Feng Chen, Yue Gao, Tong-chao Wang, Juan Song, Chen Yang, Guo-Jun Cai, Ming-Xiao Hou, Wei Zhang, Guo-bing Shi |
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| Rok vydání: | 2015 |
| Předmět: |
Male
Hot Temperature Neural Conduction Constriction Pathologic Motor Activity Pharmacology Nerve conduction velocity Rats Sprague-Dawley Necrosis Myelin Neurotrophic factors Ganglia Spinal medicine Animals Analgesics business.industry Body Weight Saponins Sciatic Nerve Triterpenes Disease Models Animal Psychiatry and Mental health Nociception medicine.anatomical_structure Allodynia Hyperalgesia Touch Anesthesia Neuropathic pain Neuralgia Sciatic nerve Chronic Pain medicine.symptom business |
| Zdroj: | Behavioural Pharmacology. 26:436-446 |
| ISSN: | 0955-8810 |
| Popis: | To investigate the applicability of astragaloside IV (AG) for the treatment of refractory neuropathic pain, we systemically evaluated the antinociceptive activity of AG in the animal model of chronic constriction injury. We studied behaviors, electrophysiology, and biochemistry from day 2 to day 23 after the surgery. We found that when administered intraperitoneally at the dose of 60 mg/kg, AG caused significant inhibition of allodynia and hyperalgesia induced by mechanic and thermal stimuli as well as downregulation of the expressions of a series of proteins involved in mediating neuropathic pain in the dorsal root ganglia, such as P2X purinoceptor 3, glial cell-derived neurotrophic factor, glial cell-derived neurotrophic factor family receptor α1, and transient receptor potential cation channel subtypes A1 and V1. Further investigation showed that AG restored the nerve conduction velocity and the histological structure of the damaged sciatic nerve on day 23 after the surgery. Moreover, results from immunoelectron microscope showed that glial cell-derived neurotrophic factor family receptor α1 induced by AG could form a circular band in the myelin debris between the injured axons and Schwann cells, contributing toward restoration of the damaged nerve. In conclusion, in our animal model, AG effectively inhibited the neuropathic pain induced by chronic constriction injury. |
| Databáze: | OpenAIRE |
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