RNA and a cell wall component of Enterococcus faecalis IC-1 are required for phagocytosis and interleukin 12 production by the mouse macrophage cell line J774.1
| Autor: | Takayoshi Kubo, Yuko M. Sagesaka, Yuuichi Ukawa, Junpei Nakase, Syoji Takemoto, Mamoru Totsuka, Ayako Aoki-Yoshida |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
RNase P Phagocytosis Oligonucleotides Gene Expression Biology Applied Microbiology and Biotechnology Biochemistry Enterococcus faecalis Analytical Chemistry Microbiology Cell Line 03 medical and health sciences Mice Ribonucleases Cell Wall Animals Receptor Molecular Biology Membrane Glycoproteins Macrophages Organic Chemistry RNA General Medicine TLR7 biology.organism_classification Interleukin-12 Coculture Techniques RNA Bacterial 030104 developmental biology Toll-Like Receptor 7 Cell culture Interleukin 12 Muramidase Biotechnology |
| Zdroj: | Bioscience, biotechnology, and biochemistry. 81(6) |
| ISSN: | 1347-6947 |
| Popis: | Enterococcus faecalis is a resident lactic acid bacterium in the human intestine. Its immunostimulatory action was reported to be enhanced by heat sterilization. To investigate its beneficial actions, we evaluated the ability of 10 E. faecalis strains to induce interleukin-12 (IL-12) production in a mouse macrophage cell line, J774.1 and found that the strain, E. faecalis IC-1, had a potent IL-12-inducing ability. Furthermore, we investigated the underlying mechanism by treating IC-1 cells with RNase or lysozyme. Its activity almost disappeared and an antagonist of Toll-like receptor (TLR) 7 inhibited this activity. Moreover, lysozyme-treated IC-1 bacteria were not phagocytized by J774.1 cells, and did not induce IL-12 production. Based on our results, we propose that macrophages recognize the cell wall components of IC-1, leading to phagocytosis. The IC-1 RNA is then recognized by TLR7, which induces the production of IL-12. |
| Databáze: | OpenAIRE |
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