Inheritance and structure of foreign DNA in progenies of transgenic tobacco obtained by direct gene transfer

Autor:	Hirofumi Uchimiya, H. Morota
Rok vydání:	1988
Předmět:	Genetics Genetic transfer Chromosome General Medicine Biology Genome chemistry.chemical_compound chemistry Genetic linkage Genetic marker Agronomy and Crop Science Gene DNA Biotechnology Southern blot
Zdroj:	Theoretical and Applied Genetics. 76:161-164
ISSN:	1432-2242 0040-5752
DOI:	10.1007/bf00257840
Popis:	One of the transformed tobacco plants obtained by direct DNA transformation possessed two marker genes, a chimeric aminoglycoside phosphotransferase and nopaline synthase genes. Selfed progenies of this plant (T3-d) showed stable inheritance of these two genes. The minimum size of foreign DNA integrated into tobacco genome was estimated to be 5.4 kbp. A deleted nopaline synthase gene co-existed with an intact gene. The linkage analysis indicated that two transformants, T1-b and T3-c, possessed foreign DNA inserted in different chromosomes or in different sites of the same chromosome that recombine freely.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7f6be14150f309b1dcb70e3c06704e2a https://doi.org/10.1007/bf00257840 Zobrazit plný text záznamu Full text from SpringerLink