Responsiveness of carnosine homeostasis genes in the pancreas and brain of streptozotocin-treated mice exposed to dietary carnosine
| Autor: | Stefania Ippati, Francesca Gatti, Carla Vetrugno, Amilcare Barca, Tiziano Verri, Daniela Spagnolo |
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| Přispěvatelé: | Barca, Amilcare, Gatti, Francesca, Spagnolo, Daniela, Ippati, Stefania, Vetrugno, Carla, Verri, Tiziano |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Blood Glucose Male dietary carnosine medicine.medical_treatment Carnosine Administration Oral Endogeny Diabete Catalysi lcsh:Chemistry chemistry.chemical_compound Gene expression Homeostasis Insulin Pancrea pancreas lcsh:QH301-705.5 Spectroscopy diabetes Communication Diabetes mouse model Brain Computer Science Applications1707 Computer Vision and Pattern Recognition General Medicine Computer Science Applications di-tripeptides medicine.anatomical_structure diabetes mouse models Homeostasis of small peptide Pancreas medicine.drug insulin medicine.medical_specialty Reproducibility of Result Biology Catalysis Streptozocin Tissue Extract Inorganic Chemistry 03 medical and health sciences Downregulation and upregulation Internal medicine Cndp2 dipeptidase Homeostasi medicine Animals Pept2/Slc15a2 oligopeptide transporter RNA Messenger Physical and Theoretical Chemistry Molecular Biology Tissue Extracts Animal Body Weight Organic Chemistry Reproducibility of Results Di-tripeptide homeostasis of small peptides Streptozotocin Diet Mice Inbred C57BL 030104 developmental biology Endocrinology lcsh:Biology (General) lcsh:QD1-999 chemistry Hyperglycemia hyperglycemia human activities Dietary carnosine |
| Zdroj: | International Journal of Molecular Sciences, Vol 19, Iss 6, p 1713 (2018) International Journal of Molecular Sciences |
| ISSN: | 1422-0067 |
| Popis: | In excitable tissues, the endogenous dipeptide carnosine (CAR, β-Ala-l-His) sustains homeostatic responses to various challenges. By eliciting hypoglycemic effects via actions on the autonomic nervous system and protection of pancreatic beta-cells, CAR is also relevant in diabetes. We investigated the expression of genes involved in CAR biosynthesis, degradation, and membrane transport pathways, in the pancreas and brains of mice treated with streptozotocin (STZ) and then exposed to dietary CAR. We induced hyperglycemia by STZ intraperitoneal injections; then, STZ-treated mice received drinking water with or without CAR for two weeks. We report that CAR administration elicits beneficial effects on blood glucose levels and weight loss in STZ-treated mice and, remarkably, on the insulin gene products in the pancreas, preserving gene expression from STZ challenge. Also, we describe mRNA downregulation of the Slc15a2/Pept2 (dipeptide transporter) and Cndp2 (intracellular dipeptidase) genes in the pancreas of hyperglycemic mice, and dysregulation of Carns1 (CAR synthase), Pept2 and Cndp2 in brains; interestingly, dietary CAR elicits counteracting effects. These expression patterns associate with variations of CAR content in tissues of mice. Overall, our report suggests a direct role of CAR in the diabetes-affected pancreas and in the diabetes-targeted CNS, proposing (dys)regulation of CAR’s homeostasis as a marker condition. |
| Databáze: | OpenAIRE |
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