Anticoagulant and antifibrinogenolytic properties of the aqueous extract from Bauhinia forficata against snake venoms
| Autor: | Miriam Verginia Lourenço, Suely Vilela Sampaio, Clayton Z. Oliveira, Suzelei C. França, Victor A. Maiorano, Ana Helena Januário, Silvana Marcussi, Carolina D. Sant’Ana, Andreimar M. Soares, Paulo Pereira |
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| Rok vydání: | 2005 |
| Předmět: |
Drug Evaluation
Preclinical Venom Pharmacognosy Pharmacology complex mixtures Phospholipase A2 Bauhinia forficata Crotalid Venoms Drug Discovery Botany Animals biology Plant Extracts Ophidia Anticoagulants Water Crotalus Plant Components Aerial biology.organism_classification Antifibrinolytic Agents Clotting time Bauhinia biology.protein Bothrops Electrophoresis Polyacrylamide Gel Snake Venoms |
| Zdroj: | Journal of Ethnopharmacology. 98:213-216 |
| ISSN: | 0378-8741 |
| DOI: | 10.1016/j.jep.2004.12.028 |
| Popis: | The aqueous extract from aerial parts of Bauhinia forficata was able to neutralize the clotting activity induced by Bothrops and Crotalus crude venoms. The clotting time, upon human plasma, induced by B. moojeni venom was significantly prolonged. Clotting and fibrinogenolytic activities induced by isolated thrombin-like enzyme from Bothrops jararacussu were totally inhibited after incubation at different ratios. The extract was not able to neutralize the hemorrhagic activity induced by an Bothrops venoms, but it efficiently inhibited the edema induced by Crotalus durissus terrificus venom and isolated PLA2s. In addition, it did not inhibited the phospholipase A2 activity of Bothrops snake venoms. Interaction studies between Bauhinia forficata extract and snake venoms, when analyzed by SDS-PAGE, did not reveal any apparent degradation of the venom proteins. This extract is a promising source of natural inhibitors of serine-proteases involved in blood clotting disturbances induced by snake venoms. |
| Databáze: | OpenAIRE |
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