Synthesis, biological evaluation and molecular modeling of pseudo-peptides based statine as inhibitors for human tissue kallikrein 5
| Autor: | Lucas Villas Bôas Hoelz, Marcelo B. Zani, Lucas R. de Souza, Luiza R.S. Dias, Sergio Pinheiro, Silvia G. Passos, Bruna C. Zorzanelli, Pedro H.R. de A. Azevedo, Luciano Puzer, Estela M.F. Muri |
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| Rok vydání: | 2016 |
| Předmět: |
Models
Molecular 0301 basic medicine Molecular model Peptidomimetic Tissue kallikrein 03 medical and health sciences chemistry.chemical_compound Molecular dynamics 0302 clinical medicine Drug Discovery Statine medicine Humans Amino Acids Enzyme Inhibitors Pharmacology Organic Chemistry Cancer KLK5 General Medicine Kallikrein medicine.disease 030104 developmental biology chemistry Biochemistry 030220 oncology & carcinogenesis Kallikreins Peptidomimetics |
| Zdroj: | European Journal of Medicinal Chemistry. 112:39-47 |
| ISSN: | 0223-5234 |
| Popis: | Human kallikrein 5 (KLK5) is a potential target for the treatment of skin inflammation and cancer. A new series of statine based peptidomimetic compounds were designed and synthesized through simple and efficient reactions. Some KLK5 inhibitors (2a-c compounds) were identified with nanomolar affinity showing Ki values of 0.12-0.13 μM. Our molecular modeling studies suggest that the inhibitors binding at the KLK5 through H-bond interactions with key residues (mainly His108, Gln242, Gly243, Ser245, and Ser260), disrupting the correlated motions mainly among the Ile67-Tyr127, Glu128-Val187, and Gly237-Ser293 subdomains, which seems to be crucial for KLK5 activity. Therefore, we believe that these findings will significantly facilitate our understanding of the conformational dynamics in the course of KLK5 inhibition and, consequently, the development of more potent molecules as alternative for cancer treatment. |
| Databáze: | OpenAIRE |
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