Novel Centromeric Loci of the Wine and Beer Yeast Dekkera bruxellensis CEN1 and CEN2
| Autor: | Anna Judith Schifferdecker, Åsa K. Hagström, Jure Piškur, Olena P. Ishchuk, Elżbieta Rozpędowska, Sofia Mebrahtu Wisén, Tanja Vojvoda Zeljko, Mikael Rørdam Andersen, Linda Hellborg, Zhihao Ling, Andrei A. Sibirny |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Autonomously replicating sequence Molecular biology lcsh:Medicine Yeast and Fungal Models Wine centromere karyotype variability autonomous replication aneuploidy MITE-like transposon Dekkera bruxellensis Genome Plasmid Mobile Genetic Elements Genomic library lcsh:Science Homologous Recombination Conserved Sequence Genetics Centromeres Multidisciplinary Chromosome Biology Beer Genomics Saccharomyces Cerevisiae Chromosome breakage Sequence Analysis Research Article Transposable element Chromosome Structure and Function Centromere Genes Fungal Biology DNA construction Chromosomes Genomic Instability 03 medical and health sciences Saccharomyces Genetic Elements Model Organisms Sequence Motif Analysis Sequencing Techniques Gene Ploidies Dekkera lcsh:R Transposable Elements Organisms Fungi Biology and Life Sciences Cell Biology Yeast Research and analysis methods 030104 developmental biology Molecular biology techniques Genetic Loci Biofilms Plasmid Construction lcsh:Q |
| Zdroj: | Ishchuk, O P, Vojvoda Zeljko, T, Schifferdecker, A J, Mebrahtu Wisén, S, Hagström, Å K, Rozpędowska, E, Andersen, M R, Hellborg, L, Ling, Z, Sibirny, A A & Piškur, J 2016, ' Novel Centromeric Loci of the Wine and Beer Yeast Dekkera bruxellensis CEN1 and CEN2 ', P L o S One, vol. 11, no. 8, e0161741 . https://doi.org/10.1371/journal.pone.0161741 PLoS ONE PLoS ONE, Vol 11, Iss 8, p e0161741 (2016) |
| Popis: | The wine and beer yeast Dekkera bruxellensis thrives in environments that are harsh and limiting, especially in concentrations with low oxygen and high ethanol. Its different strains' chromosomes greatly vary in number (karyotype). This study isolates two novel centromeric loci (CEN1 and CEN2), which support both the yeast's autonomous replication and the stable maintenance of plasmids. In the sequenced genome of the D. bruxellensis strain CBS 2499, CEN1 and CEN2 are each present in one copy. They differ from the known "point" CEN elements, and their biological activity is retained within ~900-1300 bp DNA segments. CEN1 and CEN2 have features of both "point" and "regional" centromeres: They contain conserved DNA elements, ARSs, short repeats, one tRNA gene, and transposon-like elements within less than 1 kb. Our discovery of a miniature inverted-repeat transposable element (MITE) next to CEN2 is the first report of such transposons in yeast. The transformants carrying circular plasmids with cloned CEN1 and CEN2 undergo a phenotypic switch: They form fluffy colonies and produce three times more biofilm. The introduction of extra copies of CEN1 and CEN2 promotes both genome rearrangements and ploidy shifts, with these effects mediated by homologous recombination (between circular plasmid and genome centromere copy) or by chromosome breakage when integrated. Also, the proximity of the MITE-like transposon to CEN2 could translocate CEN2 within the genome or cause chromosomal breaks, so promoting genome dynamics. With extra copies of CEN1 and CEN2, the yeast's enhanced capacities to rearrange its genome and to change its gene expression could increase its abilities for exploiting new and demanding niches. |
| Databáze: | OpenAIRE |
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