Lack of pathogenicity and toxicity of the mycoinsecticide Metarhizium anisopliae var. acridum following acute gastric exposure in mice
| Autor: | Víctor Manuel Hernández-Velázquez, Marte Lorenzana-Jiménez, Conchita Toriello, Armando Pérez-Torres, Teresa Mier, Amelia Pérez-Mejía, Hortensia Navarro-Barranco, Fabiola Vega-García |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Metarhizium
medicine.medical_specialty Health Toxicology and Mutagenesis Administration Oral Metarhizium anisopliae Grasshoppers Fungus Microbiology Conidium Gross examination Mice Toxicity Tests Acute medicine Animals Pest Control Biological biology Inoculation Stomach fungi Public Health Environmental and Occupational Health General Medicine Spores Fungal biology.organism_classification Pollution medicine.anatomical_structure Liver Mycoses Splenomegaly Toxicity Histopathology |
| Zdroj: | Ecotoxicology and Environmental Safety. 72:2153-2157 |
| ISSN: | 0147-6513 |
| DOI: | 10.1016/j.ecoenv.2009.05.014 |
| Popis: | Metarhizium anisopliae var. acridum, monospore culture EH-502/8 (CNRCB MaPL40), isolated in Mexico from Schistocerca piceifrons ssp. piceifrons (Orthoptera: Acrididae) was tested for acute oral intragastric pathogenicity and toxicity in CD-1 mice. Animals were inoculated with one dose (108 conidia/animal) of viable (72 mice), non-viable (24 mice) conidia and compared to 18 control mice. Clinical observations were done daily; mycological and histological tests were performed during necropsies after the inoculation. No mice showed clinical symptoms of illness or died during the study. The fungus was able to persist in some organs until day 3, but did not cause any damage to the host. The gross pathology observed was splenomegaly in mice inoculated with viable and non-viable conidia. Non-germinated conidia, observed in several organs, suggest hematogenous spread, but without any histopathological tissue reaction. Results support the non-pathogenic and non-toxic status of this fungal strain when administered in a single intragastric dose to mice. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect