Harvesting multipotent progenitor cells from a small sample of tonsillar biopsy for clinical applications
| Autor: | Ying Wang, Raju Khatri, Michal Arad, Benjamin A. Portney, Michal Zalzman, Timothy Ortlip, David M. Kaetzel, Rodney J. Taylor, Silviu C. Diaconu, W. Alex Meltzer, Lorna E. Silipino |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
Male
0301 basic medicine Pathology medicine.medical_specialty Biopsy Palatine Tonsil Medicine (miscellaneous) Clinical uses of mesenchymal stem cells Cell Separation Biology Biochemistry Genetics and Molecular Biology (miscellaneous) Regenerative medicine Cell therapy lcsh:Biochemistry 03 medical and health sciences Multipotency medicine Humans Mesenchymal progenitor cells lcsh:QD415-436 Progenitor cell Induced pluripotent stem cell lcsh:R5-920 medicine.diagnostic_test Research Multipotent Stem Cells Mesenchymal stem cell Cell Biology 030104 developmental biology Tonsil Molecular Medicine Female Stem cell lcsh:Medicine (General) Adult stem cell |
| Zdroj: | Stem Cell Research & Therapy, Vol 8, Iss 1, Pp 1-11 (2017) Stem Cell Research & Therapy |
| ISSN: | 1757-6512 |
| DOI: | 10.1186/s13287-017-0619-x |
| Popis: | Background Human adult stem cells hold the potential for the cure of numerous conditions and degenerative diseases. They possess major advantages over pluripotent stem cells as they can be derived from donors at any age, and therefore pose no ethical concerns or risk of teratoma tumor formation in vivo. Furthermore, they have a natural ability to differentiate and secrete factors that promote tissue healing without genetic manipulation. However, at present, clinical applications of adult stem cells are limited by a shortage of a reliable, standardized, and easily accessible tissue source which does not rely on specimens discarded from unrelated surgical procedures. Method Human tonsil-derived mesenchymal progenitor cells (MPCs) were isolated from a small sample of tonsillar tissue (average 0.88 cm3). Our novel procedure poses a minimal mechanical and enzymatic insult to the tissue, and therefore leads to high cell viability and yield. We characterized these MPCs and demonstrated robust multipotency in vitro. We further show that these cells can be propagated and maintained in xeno-free conditions. Results We have generated tonsillar biopsy-derived MPC (T-MPC) lines from multiple donors across a spectrum of age, sex, and race, and successfully expanded them in culture. We characterized them by cell surface markers, as well as in vitro expansion and differentiation potential. Our procedure provides a robust yield of tonsillar biopsy-derived T-MPCs. Conclusions Millions of MPCs can be harvested from a sample smaller than 1 g, which can be collected from a fully awake donor in an outpatient setting without the need for general anesthesia or hospitalization. Our study identifies tonsillar biopsy as an abundant source of adult MPCs for regenerative medicine. Electronic supplementary material The online version of this article (doi:10.1186/s13287-017-0619-x) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
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