Transforming growth factor-beta stimulates, and glucocorticoids and epidermal growth factor inhibit brain natriuretic peptide secretion from cultured human amnion cells
| Autor: | T Mori, Norimasa Sagawa, Hiroshi Ueda, Fuminori Kobayashi, H. Nanno, M. Hasegawa, Yu Ihara, K. Kitagawa, K. Inamori, H Itoh |
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| Rok vydání: | 1994 |
| Předmět: |
medicine.medical_specialty
Hydrocortisone Endocrinology Diabetes and Metabolism Clinical Biochemistry Nerve Tissue Proteins Biology Biochemistry Endocrinology Pregnancy Transforming Growth Factor beta Epidermal growth factor Internal medicine Natriuretic Peptide Brain TGF beta signaling pathway medicine Humans Secretion Amnion Cells Cultured Epidermal Growth Factor Biochemistry (medical) Transforming growth factor beta Amniotic Fluid Brain natriuretic peptide medicine.anatomical_structure Cell culture biology.protein Female hormones hormone substitutes and hormone antagonists Transforming growth factor |
| Zdroj: | The Journal of Clinical Endocrinology & Metabolism. 79:176-182 |
| ISSN: | 1945-7197 0021-972X |
| DOI: | 10.1210/jcem.79.1.8027223 |
| Popis: | We previously reported the massive secretion of brain natriuretic peptide (BNP) from human amnion cells and suggested the possible role of BNP in the maintenance of human pregnancy. In this study, to elucidate the regulatory mechanism of BNP secretion from amnion cells, we measured the BNP level in the culture medium of amnion cells by RIA after incubation in the presence of various substances. Among the agents examined, cortisol (1 x 10(-7) to 1 x 10(-6) mol/L), dexamethasone (1 x 10(-8) to 1 x 10(-6) mol/L), and epidermal growth factor (EGF; 2 x 10(-11) to 2 x 10(-8) mol/L) inhibited BNP secretion from the cultured amnion cells in a dose-dependent manner. By contrast, transforming growth factor-beta (TGF beta; 4 x 10(-11) to 4 x 10(-9) mol/L) caused a 3- to 5-fold increase in BNP secretion. TGF beta-augmented BNP secretion was abolished by the addition of cortisol or EGF to the culture medium. Moreover, in this study, we revealed the presence of bioactive TGF beta in human amniotic fluid (approximately 4 x 10(-10) mol/L). The present finding of tight regulation of BNP secretion from amnion cells by cortisol, EGF and TGF beta, all at the concentrations physiologically present in human amniotic fluid, implies a physiological role of BNP secretion from amnion cells in the pregnant uterus. |
| Databáze: | OpenAIRE |
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