Qualitative and quantitative analyses of aconite alkaloids in Aconiti kusnezoffii Radix, and NO inhibitory activity evaluation of the alkaloid extracts
| Autor: | Jia-Xin Zhu, Qin-Yu Li, Na Zhang, Chun-Hong Zhang, Wen-Shuo Wang, Xue Gong, Bo Wu |
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| Rok vydání: | 2022 |
| Předmět: |
ultrahigh performance liquid chromatography-electrospray ionization tandem mass spectrometry
Traditional medicine biology Nutrition. Foods and food supply Electrospray ionization Alkaloid aconite alkaloid biology.organism_classification High-performance liquid chromatography qualitative and quantitative analyses chemistry.chemical_compound Qualitative analysis chemistry anti-inflammatory effect Chromatography detector T1-995 Aconitine TX341-641 Radix Aconiti kusnezoffii Radix Technology (General) Aconitum Food Science Biotechnology |
| Zdroj: | Food Science and Technology (2021) Food Science and Technology v.42 2022 Food Science and Technology (Campinas) Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA) instacron:SBCTA Food Science and Technology, Issue: ahead, Published: 28 JUN 2021 Food Science and Technology, Volume: 42, Article number: e28521, Published: 28 JUN 2021 |
| ISSN: | 1678-457X 0101-2061 |
| Popis: | Aconiti kusnezoffii Radix (Caowu), a traditional Chinese medicine and Mongolian medicine, has been commonly used owing to its analgesic and anti-inflammatory effects. Here, we performed a qualitative analysis of aconite alkaloids (AAs) in raw and processed Aconiti kusnezoffii Radix by ultra-high-performance liquid chromatography (HPLC) electrospray ionization-tandem mass spectrometry. Furthermore, we quantified benzoylaconine, benzoylmesaconine, benzoylhypaconine, aconitine, mesaconitine, and hypaconitine in raw and processed Aconiti kusnezoffii Radix using a HPLC system coupled with a diode array detector. The analysis of AA fragmentation patterns in positive electrospray ionization mode revealed 155 AAs, including 6 with unambiguous identities, 109 with putative assignments, and 40 unknown compounds. Additionally, lipopolysaccharide-treated RAW264.7 macrophages were used to evaluate the anti-inflammatory properties of the alkaloid extracts of Aconiti kusnezoffii Radix. Importantly, alkaloid extracts of Aconiti kusnezoffii Radix at three different concentrations exhibited protective effect against lipopolysaccharide-induced inflammation. Thus, the proposed method can be used not only for the qualitative and quantitative analyses of Aconiti kusnezoffii Radix, but also for evaluating other Aconitum spp. Our study lays a foundation for the clinical application of poisonous Aconitum. |
| Databáze: | OpenAIRE |
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