Mitochondrial damage and DNA depletion in cord blood and umbilical cord from infants exposed in utero to Combivir
| Autor: | Mary Ellen Adams, Nancy A. Wade, Steven K. Seilkop, Elaine J. Abrams, Vernon E. Walker, Kunio Nagashima, Rao L. Divi, J. Patrick O'Neill, Miriam C. Poirier, Carol J. Nesel |
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| Rok vydání: | 2004 |
| Předmět: |
Mitochondrial DNA
Anti-HIV Agents Immunology Physiology HIV Infections Pilot Projects Biology Umbilical cord DNA Mitochondrial Umbilical Cord Zidovudine Pregnancy medicine Immunology and Allergy Humans Pregnancy Complications Infectious Maternal-Fetal Exchange Infant Newborn Lamivudine medicine.disease Fetal Blood CD4 Lymphocyte Count Mitochondria Drug Combinations Microscopy Electron Infectious Diseases medicine.anatomical_structure In utero Cord blood Prenatal Exposure Delayed Effects HIV-1 Gestation Female medicine.drug |
| Zdroj: | AIDS (London, England). 18(7) |
| ISSN: | 0269-9370 |
| Popis: | Objective Although most uninfected infants born to women infected with HIV-1 show no clinical evidence of mitochondrial compromise, mitochondrial dysfunction has been reported in children born to women receiving zidovudine and/or lamivudine during pregnancy. In this pilot study we examined mitochondrial integrity in HIV-1-uninfected infants born to HIV-1-infected women receiving Combivir during pregnancy. Design : Samples of umbilical cord and cord blood were obtained from HIV-1-uninfected infants born to either HIV-1-infected women receiving Combivir therapy during pregnancy (n = 10) or HIV-1-uninfected women (n = 9). Methods Mitochondrial morphological integrity was examined in umbilical cords (n = 16) by electron microscopy and mtDNA quantity was determined in DNA from cord blood (n = 18) and umbilical cord (n = 18) by PCR-chemiluminescence immunoassay detection. Results In umbilical cords from six of nine infants born to HIV-1-infected mothers taking Combivir moderate to severe mitochondrial morphological damage was observed (P = 0.011), while none of seven unexposed infants showed similar damage. Compared to unexposed infants, statistically significant mtDNA depletion was observed in umbilical cord (P = 0.006) and cord blood (P = 0.003) from drug-exposed infants. Conclusions A cohort of HIV-1-uninfected Combivir-exposed infants with no clinical symptoms showed morphological and molecular evidence of mitochondrial damage. |
| Databáze: | OpenAIRE |
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