Human herpesvirus type 7 in blood donors: Detection by the polymerase chain reaction
| Autor: | R. Peng, F. Lorenz, D. Huhn, C. A. Schmidt, Wolfgang Siegert, F Wilborn, Hans R. Gelderblom |
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| Rok vydání: | 1995 |
| Předmět: |
Adult
Male Coat Molecular Sequence Data Blood Donors Herpesvirus 7 Human Buffy coat Urine Biology Polymerase Chain Reaction Sensitivity and Specificity law.invention law Virology Leukocytes Prevalence Lavage fluid Humans Longitudinal Studies Saliva Polymerase chain reaction DNA Primers Base Sequence Middle Aged Human herpesvirus type 7 Cross-Sectional Studies Infectious Diseases DNA Viral Female Viral disease Nested polymerase chain reaction |
| Zdroj: | Journal of Medical Virology. 47:65-69 |
| ISSN: | 1096-9071 0146-6615 |
| DOI: | 10.1002/jmv.1890470113 |
| Popis: | In order to evaluate the prevalence of human herpesvirus type 7 (HHV-7) in adult blood donors oral lavage fluid, bum coat, and uring samples from 112 persons were examined by the polymerase chain reaction (PCR) at one time point. In addition, 11 donors were studied longitudinally over 11 weeks. When the results of the initial and the longitudinal study were combined HHV-7 DNA was found in samples from 109 of 112 (97.3%) adult blood donors. On the basis of different sensitivity levels of the first and the nested PCR differences were detected in the viral DNA load in the samples. It was found that lavage fluid regularly carried significantly higher DNA concentrations than buffy coat. Out of 112 donors, 102 (91.1%) and 8 (7.1%) were positive in the first, less sensitive PCR in lavage fluid and buffy coat, respectively (P |
| Databáze: | OpenAIRE |
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