Clonal composition of Escherichia coli causing community-acquired urinary tract infections in the State of Rio de Janeiro, Brazil

Autor: Sherry P. Smith, Rubens Clayton da Silva Dias, Elizabeth Mendes Alves, Lee W. Riley, Denise V. Marangoni, Flávia Lúcia Piffano Costa Pellegrino, Beatriz Meurer Moreira
Rok vydání: 2009
Předmět:
Microbiology (medical)
Adult
Adolescent
Immunology
Molecular Sequence Data
Anti-Infective Agents
Urinary
Drug resistance
Biology
medicine.disease_cause
urologic and male genital diseases
Microbiology
Young Adult
Intergenic region
Ampicillin
Drug Resistance
Multiple
Bacterial
Trimethoprim
Sulfamethoxazole Drug Combination
medicine
Outpatient clinic
Humans
Uropathogenic Escherichia coli
Disease
Prospective Studies
Escherichia coli
Escherichia coli Infections
Aged
Pharmacology
Aged
80 and over
Sulfamethoxazole
Middle Aged
bacterial infections and mycoses
Trimethoprim
female genital diseases and pregnancy complications
Anti-Bacterial Agents
Bacterial Typing Techniques
Electrophoresis
Gel
Pulsed-Field
Cross-Sectional Studies
Urinary Tract Infections
Multilocus sequence typing
Female
Brazil
medicine.drug
Zdroj: Microbial drug resistance (Larchmont, N.Y.). 15(4)
ISSN: 1931-8448
Popis: Recent studies from North America and Europe have demonstrated community-wide clonal spread of uropathogenic Escherichia coli (UPEC). To investigate if a similar pattern of spread occurs in Brazil, we characterized UPEC from women with community-acquired urinary tract infection (UTI) in Rio de Janeiro. E. coli isolates from women with UTI in one public outpatient clinic were evaluated for antibiotic susceptibility, E. coli phylogenetic grouping, enterobacterial repetitive intergenic consensus (ERIC) 2 PCR and pulsed-field gel electrophoresis fingerprinting, and multilocus sequence typing. From March 2005 to November 2006, 344 patients were studied. Of these, 186 (54%) had confirmed UTI, 118 (63.4%) of which were caused by E. coli. More than 50% of these isolates were resistant to ampicillin and trimethoprim/sulfamethoxazole. Of these, 96 (81%) belonged to 19 ERIC2 clonal groups. The largest group included 15 isolates, all belonging to multilocus sequence typing group ST69 and phylogenetic group D; they had pulsed-field gel electrophoresis patterns sharing at least 89% similarity compared with the CgA reference strain ATCC BAA-457. CgA strains have been found to be widespread in the United States in the early 2000s. Clonal group E. coli strains accounted for a large proportion (52%) of all UTIs and 82% of the trimethoprim/sulfamethoxazole-resistant E. coli UTIs. Thus, as in North America and Europe, UPECs that cause UTI in Rio de Janeiro also show clonal distribution, and a substantial proportion of drug-resistant UTI is caused by a small set of genetically related E. coli strains.
Databáze: OpenAIRE
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