Utilization of Amylose−Lipid Complexes as Molecular Nanocapsules for Conjugated Linoleic Acid
| Autor: | Eyal Shimoni, Hagit Bar, Imad Zakaria, Inbal Lalush, Sigal Eichler |
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| Rok vydání: | 2004 |
| Předmět: |
Time Factors
Polymers and Plastics Conjugated linoleic acid Linoleic acid Bioengineering Calorimetry Microscopy Atomic Force Nanocapsules Linoleic Acid Biomaterials chemistry.chemical_compound Hydrolysis X-Ray Diffraction Amylose Materials Chemistry Organic chemistry Amylase Pancreas Calorimetry Differential Scanning integumentary system biology Chemistry Dimethyl sulfoxide Temperature food and beverages Lipids Nanostructures Amylases biology.protein Hydrochloric Acid Oxidation-Reduction Nuclear chemistry |
| Zdroj: | Biomacromolecules. 6:121-130 |
| ISSN: | 1526-4602 1525-7797 |
| DOI: | 10.1021/bm049644f |
| Popis: | Amylose-conjugated linoleic acid (CLA) complexes were produced by water/dimethyl sulfoxide (DMSO) and KOH/HCl complexation methods. The formation of amylose V form was confirmed by X-ray diffraction (XRD), and complexes formed at 30, 60, and 90 degrees C exhibit melting temperatures exceeding 88 degrees C. Atomic force microscopy (AFM) images showed distinct difference in complex organization, with complexes formed in water/DMSO showing spherical shape with typical diameter of 150 nm. Complexes formed by KOH/HCl showed elongated structure with typical width of 43-160 nm. Water/DMSO complexes exhibit superior protection to CLA against oxidation. All complexes showed high retention of CLA in simulated stomach conditions, and the digestion of complexes by amylases results in high hydrolysis and CLA release by pancreatin and alpha-amylase. Only moderate release was detected following hydrolysis by amyloglucosidase and beta-amylase. It is therefore suggested that amylose-CLA complexes can serve as molecular nanocapsules for protection and delivery of CLA. |
| Databáze: | OpenAIRE |
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