RHD positive among C/E+ and D-negative blood donors in Tunisia
| Autor: | Hajer Moussa, Jean Gabert, T. Chakroun, Marthe Tsochandaridis, S. Abdelkefi, A. Levy, Narjes Kacem, S. Jemni Yacoub |
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| Rok vydání: | 2014 |
| Předmět: |
Adult
Tunisia Genotype Clinical Biochemistry RhD positive Blood Donors Biology Rh Isoimmunization Exon Gene Frequency Antigen Isoantibodies Humans Allele Genotyping Gene Alleles Sequence Deletion Genetics Rh-Hr Blood-Group System Biochemistry (medical) Exons Sequence Analysis DNA Hematology Virology Phenotype Real-time polymerase chain reaction Blood Grouping and Crossmatching Variants of PCR Gene Deletion |
| Zdroj: | Transfusion Clinique et Biologique. 21:320-323 |
| ISSN: | 1246-7820 |
| Popis: | Purpose of the study The aim of this study was to investigate RHD alleles among Tunisian blood donors with D-negative phenotype and positive for C and/or E antigen. Patients and methods A total of 100 D-negative and C/E+ samples were analyzed by RHD genotyping using an initial test for RHD exon 10. In case of a positive reaction, further molecular investigations including real time quantitative PCR, allele specific PCR and nucleotide sequencing were done to elucidate the RHD involved mechanisms. Results Seventy-five percent of the studied samples lacked the RHD gene. Twenty-three percent carried the hybrid RHD-CE-D alleles (16 RHD-CE(3-7)-D , 5 RHD-CE(4-7)-D , 1 RHD-CE(4-8)-D , 1 RHD-CE(3-8)-D ) and 2% were weak D (1 weak D type 1 and 1 weak D type 5). Conclusion Our study proved the high frequency of RHD gene among serologically D-negative samples, positive for C and/or E antigen. Thus achieving systematically RHCE phenotyping in all transfusion centers on the Tunisian territory and considering blood donated from D-negative C/E+ persons as D-positive will be recommended to reduce anti-D allo-immunization. |
| Databáze: | OpenAIRE |
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