Evaluation of Echinococcus granulosus recombinant EgAgB8/1, EgAgB8/2 and EPC1 antigens in the diagnosis of cystic echinococcosis in buffaloes
| Autor: | O.K. Raina, Andleeb Aftab, S. Samanta, Mary Nisha Tigga, Anju Varghese, Praveen Singh, M. Norjit Singh, P.S. Banerjee, Arun Anandanarayanan, Ajayta Rialch, M. R. Verma, H. Lalrinkima, Zamir Ali Ganaie |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Buffaloes Fasciola gigantica 030231 tropical medicine Antibodies Helminth Enzyme-Linked Immunosorbent Assay medicine.disease_cause Sensitivity and Specificity Cross-reactivity law.invention Microbiology 03 medical and health sciences 0302 clinical medicine Antigen Echinococcosis law parasitic diseases medicine Animals Echinococcus granulosus General Veterinary biology Helminth Proteins General Medicine biology.organism_classification medicine.disease Recombinant Proteins 030104 developmental biology Echinococcus Antigens Helminth Immunoglobulin G Sarcocystis Recombinant DNA Parasitology |
| Zdroj: | Veterinary Parasitology. 252:29-34 |
| ISSN: | 0304-4017 |
| Popis: | Three recombinant proteins of Echinococcus granulosus including two antigen B sub-units EgAgB8/1 and EgAgB8/2 and Echinococcus protoscolex calcium binding protein 1 (EPC1) were expressed in prokaryotic expression vectors. The diagnostic potential of these three recombinant proteins was evaluated in the detection of cystic echinococcosis in buffaloes in IgG-ELISA. The EgAgB8/1 and EgAgB8/2 recombinant proteins reacted fairly with the hydatid infected buffaloes with sensitivity of 75.0% and 78.6%, respectively and specificity of 75.8% while EPC1 recombinant protein showed higher sensitivity (89.3%) but lower specificity (51.5%). Cross-reactivity of these three antigens was assayed with buffalo sera naturally infected with Explanatum explanatum, Paramphistomum epiclitum, Gastrothylax spp., Fasciola gigantica and Sarcocystis spp. EgAgB8/1 and EPC1 antigens cross-reacted with all these sera while EgAgB8/2 showed no cross-reaction with Sarcocystis spp. and reacted with some of the E. explanatum infected buffalo sera. This study explores the potential of three hydatid antigens viz. EgAgB8/1, EgAgB8/2 and EPC1 for their diagnostic potential in buffaloes positive for cystic echinococcosis. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect