Engineering a mevalonate pathway in Escherichia coli for production of terpenoids
| Autor: | Jay D. Keasling, Vincent J. J. Martin, Sydnor T. Withers, Jack D. Newman, Douglas J. Pitera |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Amorpha-4
11-diene Biomedical Engineering Mevalonic Acid Bioengineering Isoprene synthase Alkenes Biology Protein Engineering Sesquiterpene Applied Microbiology and Biotechnology Metabolic engineering chemistry.chemical_compound Escherichia coli medicine Artemisinin Terpenes Caryophyllene fungi Gene Expression Regulation Bacterial Terpenoid chemistry Biochemistry biology.protein Molecular Medicine Mevalonate pathway Energy Metabolism Genetic Engineering Cell Division Biotechnology medicine.drug |
| Zdroj: | Nature Biotechnology. 21:796-802 |
| ISSN: | 1546-1696 1087-0156 |
| Popis: | Isoprenoids are the most numerous and structurally diverse family of natural products. Terpenoids, a class of isoprenoids often isolated from plants, are used as commercial flavor and fragrance compounds and antimalarial or anticancer drugs. Because plant tissue extractions typically yield low terpenoid concentrations, we sought an alternative method to produce high-value terpenoid compounds, such as the antimalarial drug artemisinin, in a microbial host. We engineered the expression of a synthetic amorpha-4,11-diene synthase gene and the mevalonate isoprenoid pathway from Saccharomyces cerevisiae in Escherichia coli. Concentrations of amorphadiene, the sesquiterpene olefin precursor to artemisinin, reached 24 microg caryophyllene equivalent/ml. Because isopentenyl and dimethylallyl pyrophosphates are the universal precursors to all isoprenoids, the strains developed in this study can serve as platform hosts for the production of any terpenoid compound for which a terpene synthase gene is available. |
| Databáze: | OpenAIRE |
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