Physiological characteristics, dry matter, and active component accumulation patterns of Changium smyrnioides in response to a light intensity gradient
| Autor: | Bo-xing Cheng, Zaibiao Zhu, Qiao-Sheng Guo, Chang-Lin Wang |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine Time Factors Pharmaceutical Science net photosynthetic rate 01 natural sciences Plant Roots Antioxidants chemistry.chemical_compound Drug Discovery Biomass Photosynthesis Chromatography High Pressure Liquid Plant Proteins biology General Medicine Malondialdehyde Enzymes Horticulture Spectrophotometry Sunlight Molecular Medicine Mannitol Changium medicine.drug Context (language use) 03 medical and health sciences choline Polysaccharides Botany medicine Dry matter Pharmacology Plants Medicinal lcsh:RM1-950 mannitol Water Dose-Response Relationship Radiation biology.organism_classification Light intensity lcsh:Therapeutics. Pharmacology 030104 developmental biology Complementary and alternative medicine chemistry Seedlings polysaccharide 010606 plant biology & botany Apiaceae Phytotherapy |
| Zdroj: | Pharmaceutical Biology, Vol 55, Iss 1, Pp 581-589 (2017) |
| ISSN: | 1744-5116 |
| Popis: | Context: Changium smyrnioides Wolff (Apiaceae) is an endangered medicinal plant with numerous pharmacological uses. Objective: To investigate the effect of light intensity levels on the growth and accumulation of secondary metabolites of C. smyrnioides, cultivated seedlings were subjected to different relative light intensities via sun-shading. Materials and methods: Changium smyrnioides seedlings were subjected to five irradiance treatments (100, 60.54, 44.84, 31.39, and 10.56% sunlight) in glasshouse for 9 months. Enzymatic and non-enzymatic antioxidants with spectrophotometric method, photosynthetic parameters with Li-6400XT, dry matter accumulation and active component contents in the root with spectrophotometric and HPLC method were analyzed. Results: With an increase in relative light intensity levels, activities of enzymatic and non-enzymatic antioxidants, and malondialdehyde (MDA) contents were increased overall, while net photosynthetic rate (Pn) and dry matter accumulation patter first increased and then declined. The highest net photosynthetic rate (30.68 μmol/m2·s) and dry root weight (5.07 g) were achieved under 60.54% sunlight. Lower relative light intensity levels stimulated the accumulation levels of bioactive compounds in the roots so that the highest contents of mannitol (1.35%) and choline (405.58 μg/g) were recorded under 31.39% sunlight, and the highest polysaccharide content (10.80%) were achieved under 44.84% sunlight. With a decrease in the relative light intensity levels, the water-soluble component content increased first and then decreased. Discussion and conclusion: The results revealed that 31.39–60.54% sunlight serve as appropriate relative light intensity conditions for cultivated C. smyrnioides. |
| Databáze: | OpenAIRE |
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