Effect of cumulus cells and sperm concentration on fertilization and development of pig oocytes
| Autor: | Rong Li, Henrik Callesen, Ying Liu, Hanne Skovsgaard Pedersen |
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| Rok vydání: | 2018 |
| Předmět: |
Male
0301 basic medicine endocrine system Swine post-pubertal Fertilization in Vitro Biology Andrology 03 medical and health sciences 0302 clinical medicine Endocrinology Human fertilization Post-pubertal Animals reproductive and urinary physiology Cumulus Cells 030219 obstetrics & reproductive medicine urogenital system fungi Embryogenesis swine Polyspermy Spermatozoa pronuclear formation Sperm 030104 developmental biology IVF embryonic structures Oocytes Animal Science and Zoology polyspermy Penetration rate Biotechnology |
| Zdroj: | Li, R, Liu, Y, Pedersen, H S & Callesen, H 2018, ' Effect of cumulus cells and sperm concentration on fertilization and development of pig oocytes ', Reproduction in Domestic Animals, vol. 53, pp. 1009-1012 . https://doi.org/10.1111/rda.13184 |
| ISSN: | 0936-6768 |
| DOI: | 10.1111/rda.13184 |
| Popis: | In pigs, in vitro fertilization (IVF) is still hampered by low blastocyst rates, first of all due to a high rate of polyspermy. The many attempts made to reduce this problem have often compromised penetration and fertilization rates, not improving overall developmental efficiency. Over the last decades, however, it has been convincingly demonstrated that in vitro maturation (IVM) and culture (IVC) are functioning well as part of somatic cell nuclear transfer (Liu et al., 2015). Therefore, it seems relevant to re‐evaluate the challenges in porcine in vitro embryo production with particular focus on the IVF part (cumulus cells, sperm concentration) and the oocyte source (gilt, sow). Thus, our study aimed (i) to determine the effect of cumulus cells and sperm concentration on (A) penetration and pronuclear formation as well as (B) further developmental competence in a porcine IVP system, resulting in selection of an optimized IVP protocol; (ii) to use this optimized IVP protocol to determine the developmental competence of oocytes from gilts and sows. |
| Databáze: | OpenAIRE |
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