Putative Porin of Bradyrhizobium sp. ( Lupinus ) Bacteroids Induced by Glyphosate
| Autor: | Mario García de Lacoba, M. Rosario de Felipe, Alfonso González-Sama, Nuria de María, M. Teresa Serra, Mercedes Fernández-Pascual, Isabel García-Luque, Ángeles Guevara |
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| Rok vydání: | 2007 |
| Předmět: |
Models
Molecular Molecular Sequence Data Protein domain Glycine Porins Applied Microbiology and Biotechnology Bradyrhizobium Protein Structure Secondary Lupinus Plant Microbiology Bacterial Proteins Sequence Analysis Protein Amino Acid Sequence RNA Messenger Gene Peptide sequence Phylogeny chemistry.chemical_classification Ecology biology food and beverages Fabaceae Gene Expression Regulation Bacterial Blotting Northern biology.organism_classification Molecular biology Amino acid Molecular Weight Blotting Southern chemistry Biochemistry Porin bacteria Electrophoresis Polyacrylamide Gel Food Science Biotechnology |
| Zdroj: | Applied and Environmental Microbiology. 73:5075-5082 |
| ISSN: | 1098-5336 0099-2240 |
| DOI: | 10.1128/aem.00392-07 |
| Popis: | Application of glyphosate ( N -[phosphonomethyl] glycine) to Bradyrhizobium sp. ( Lupinus )-nodulated lupin plants caused modifications in the protein pattern of bacteroids. The most significant change was the presence of a 44-kDa polypeptide in bacteroids from plants treated with the higher doses of glyphosate employed (5 and 10 mM). The polypeptide has been characterized by the amino acid sequencing of its N terminus and the isolation and nucleic acid sequencing of its encoding gene. It is putatively encoded by a single gene, and the protein has been identified as a putative porin. Protein modeling revealed the existence of several domains sharing similarity to different porins, such as a transmembrane beta-barrel. The protein has been designated BLpp, for Bradyrhizobium sp. ( Lupinus ) putative porin, and would be the first porin described in Bradyrhizobium sp. ( Lupinus ). In addition, a putative conserved domain of porins has been identified which consists of 87 amino acids, located in the BLpp sequence 30 amino acids downstream of the N-terminal region. In bacteroids, mRNA of the BLpp gene shows a basal constitutive expression that increases under glyphosate treatment, and the expression of the gene is seemingly regulated at the transcriptional level. By contrast, in free-living bacteria glyphosate treatment leads to an inhibition of BLpp mRNA accumulation, indicating a different effect of glyphosate on BLpp gene expression in bacteroids and free-living bacteria. The possible role of BLpp in a metabolite interchange between Bradyrhizobium and lupin is discussed. |
| Databáze: | OpenAIRE |
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