Antimycotic Activity and Genotoxic Evaluation of Citrus sinensis and Citrus latifolia Essential Oils
| Autor: | Jaime Sánchez-Navarrete, Julia D. Toscano-Garibay, Mario Adán Moreno-Eutimio, Myriam Arriaga-Alba, Marisela González-Avila, Nancy J. Ruiz-Pérez, Teresa Sandoval-Hernández |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Antifungal Agents Microbial Sensitivity Tests Article Gas Chromatography-Mass Spectrometry Ames test law.invention Candida tropicalis 03 medical and health sciences 0404 agricultural biotechnology law Candida albicans Botany Oils Volatile Humans Plant Oils Agar diffusion test Essential oil Multidisciplinary biology Candida glabrata Traditional medicine Candida lusitaniae Candidiasis Epithelial Cells 04 agricultural and veterinary sciences Flow Cytometry biology.organism_classification 040401 food science 030104 developmental biology Citrus × sinensis Citrus sinensis |
| Zdroj: | Scientific Reports |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/srep25371 |
| Popis: | The aim of this study was to evaluate the antifungal activity of essential oils (EOs) of Citrus sinensis (C. sinensis) and Citrus latifolia (C. latifolia) against five Candida species: Candida albicans, Candida tropicalis, Candida glabrata, Candida lusitaniae and Candida guilliermondii; and perform its genotoxic evaluation. The EOs of C. sinensis and C. latifolia were obtained from the peel by hydro-distillation. The major components determined by GC-MS were in C. sinensis, d-limonene (96%) and α-myrcene (2.79%); and in C. latifolia, d-limonene (51.64%), β-thujene (14.85%), β-pinene (12.79%) and γ-terpinene (12.8%). Antifungal properties were studied by agar diffusion method, where C. sinensis presented low activity and C. latifolia essential oil was effective to inhibit growing of C. lusitaniae and C. guilliermondii with IC50 of 6.90 and 2.92 μg respectively. The minimum inhibitory concentrations (MIC) for C. sinensis were in a range of 0.42–3.71 μg and for C. latifolia of 0.22–1.30 μg. Genotoxic evaluation was done by Ames test where none of the oils induced point mutations. Flow cytometry was used to measure toxicity in human oral epithelial cells, C. sinensis was not cytotoxic and C. latifolia was toxic at 21.8 μg. These properties might bestow different odontological applications to each essential oil. |
| Databáze: | OpenAIRE |
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