Medicago truncatula Yellow Stripe‐Like7 encodes a peptide transporter participating in symbiotic nitrogen fixation
| Autor: | Patricia Gil-Díez, Rosario Castro-Rodríguez, Ella M. Brear, Jiangqi Wen, Louis Grillet, Rakesh Kumar, Penelope M. C. Smith, María Reguera, Julia Quintana, Kirankumar S. Mysore, Viviana Escudero, Juan Imperial, Manuel González-Guerrero, Rosa Isabel Prieto, Elsbeth L. Walker |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
0301 basic medicine Root nodule Physiology Mutant Plant Science Plant Roots 01 natural sciences Rhizobia 03 medical and health sciences chemistry.chemical_compound Gene Expression Regulation Plant Nitrogen Fixation Medicago truncatula Nicotianamine Symbiosis Plant Proteins biology Cell Membrane food and beverages Plants Genetically Modified biology.organism_classification Complementation Protein Transport 030104 developmental biology chemistry Biochemistry Peptide transport Mutation Nitrogen fixation Root Nodules Plant Rhizobium 010606 plant biology & botany |
| Zdroj: | Plant, Cell & Environment. 44:1908-1920 |
| ISSN: | 1365-3040 0140-7791 |
| Popis: | Yellow Stripe-Like (YSL) proteins are a family of plant transporters that are typically involved in transition metal homeostasis. Three of the four YSL clades (I, II and IV) transport metals complexed with the non-proteinogenic amino acid nicotianamine or its derivatives. No such capability has been shown for any member of clade III, but the link between these YSLs and metal homeostasis could be masked by functional redundancy. We studied the role of the clade III YSL protein MtSYL7 in Medicago truncatula nodules. MtYSL7, which encodes a plasma membrane-bound protein, is mainly expressed in the pericycle and cortex cells of the root nodules. Yeast complementation assays revealed that MtSYL7 can transport short peptides. M. truncatula transposon insertion mutants with decreased expression of MtYSL7 had lower nitrogen fixation rates and showed reduced plant growth whether grown in symbiosis with rhizobia or not. YSL7 mutants accumulated more copper and iron in the nodules, which is likely to result from the increased expression of iron uptake and delivery genes in roots. Taken together, these data suggest that MtYSL7 plays an important role in the transition metal homeostasis of nodules and symbiotic nitrogen fixation. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text