Activity-regulated RNA editing in select neuronal subfields in hippocampus
| Autor: | Andrew C. Penn, Ales Balik, Ingo H. Greger, Zsofia Nemoda |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
Adenosine Deaminase
RNA-binding protein AMPA receptor Biology Hippocampal formation Hippocampus Rats Sprague-Dawley QH301 Genetics Animals Receptors AMPA Receptor Gene CA1 Region Hippocampal Cells Cultured Neurons Alternative splicing RNA-Binding Proteins Cell biology Rats Alternative Splicing nervous system RNA editing RNA splicing RC0321 RNA RNA Editing |
| Zdroj: | Nucleic Acids Research |
| ISSN: | 1362-4962 0305-1048 |
| Popis: | RNA editing by adensosine deaminases is a widespread mechanism to alter genetic information in metazoa. In addition to modifications in non-coding regions, editing contributes to diversification of protein function, in analogy to alternative splicing. However, although splicing programs respond to external signals, facilitating fine tuning and homeostasis of cellular functions, a similar regulation has not been described for RNA editing. Here, we show that the AMPA receptor R/G editing site is dynamically regulated in the hippocampus in response to activity. These changes are bi-directional, reversible and correlate with levels of the editase Adar2. This regulation is observed in the CA1 hippocampal subfield but not in CA3 and is thus subfield/celltype-specific. Moreover, alternative splicing of the flip/flop cassette downstream of the R/G site is closely linked to the editing state, which is regulated by Ca(2+). Our data show that A-to-I RNA editing has the capacity to tune protein function in response to external stimuli. |
| Databáze: | OpenAIRE |
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