A colorimetric, ratiometric and water-soluble fluorescent probe for simultaneously sensing glutathione and cysteine/homocysteine
| Autor: | Zhao-Yang Wang, Miao Junying, Bao-Xiang Zhao, Jie Cui, Zhi-Fang Du, Xi Dai |
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| Rok vydání: | 2015 |
| Předmět: |
Detection limit
Chromatography Homocysteine Glutathione Hydrogen-Ion Concentration Coumarin Biochemistry Fluorescence Analytical Chemistry chemistry.chemical_compound Water soluble Spectrometry Fluorescence chemistry Environmental Chemistry Colorimetry Spectrophotometry Ultraviolet Cysteine Spectroscopy Fluorescent Dyes |
| Zdroj: | Analytica chimica acta. 900 |
| ISSN: | 1873-4324 |
| Popis: | A chlorinated coumarin-aldehyde was developed as a colorimetric and ratiometric fluorescent probe for distinguishing glutathione (GSH), cystenine (Cys) and homocysteine (Hcy). The GSH-induced substitution-cyclization and Cys/Hcy-induced substitution-rearrangement cascades lead to the corresponding thiol-coumarin-iminium cation and amino-coumarin-aldehyde with distinct photophysical properties. The probe can be used to simultaneously detect GSH and Cys/Hcy by visual determination based on distinct different colors - red and pale-yellow in PBS buffer solution by two reaction sites. From the linear relationship of fluorescence intensity and biothiols concentrations, it was determined that the limits of detection for GSH, Hcy and Cys are 0.08, 0.09 and 0.18 μM, respectively. Furthermore, the probe was successfully used in living cell imaging with low cell toxicity. |
| Databáze: | OpenAIRE |
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