Effects of renal denervation on renal pelvic contractions and connexin expression in rats
| Autor: | B. Miehe, Antje Steinbach, Anna Koenen, Olaf Grisk, B. Kurt, Rainer Rettig, Uwe Zimmermann, Katrin Schaper |
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| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Male medicine.medical_specialty Contraction (grammar) Physiology Connexin Diuresis Fluorescent Antibody Technique 030204 cardiovascular system & hematology Biology Kidney Real-Time Polymerase Chain Reaction Connexins 03 medical and health sciences 0302 clinical medicine Internal medicine medicine Animals Humans Kidney Pelvis Rats Wistar Denervation Electrical impedance myography Electromyography Gap junction Muscle Smooth Rats Transplantation 030104 developmental biology Endocrinology medicine.anatomical_structure Renal pelvis Muscle Contraction |
| Zdroj: | Acta physiologica (Oxford, England). 216(2) |
| ISSN: | 1748-1716 |
| Popis: | Aims The renal pelvis shows spontaneous rhythmic contractile activity. We assessed to what extent this activity depends on renal innervation and studied the role of connexins in pelvic contractions. Methods Rats underwent unilateral renal denervation or renal transplantation. Renal pelvic pressure and diuresis were measured in vivo. Spontaneous and agonist-induced contractions of isolated renal pelves were investigated by wire myography. Rat and human renal pelvic connexin mRNA abundances and connexin localization were studied by real-time PCR and immunofluorescence respectively. Results Renal denervation or transplantation increased renal pelvic pressure in vivo by about 60 and 150%, respectively, but did not significantly affect pelvic contraction frequency. Under in vitro conditions, isolated pelvic preparations from innervated or denervated kidneys showed spontaneous contractions. Pelves from denervated kidneys showed about 50% higher contraction frequencies than pelves from innervated kidneys, whereas contraction force was similar in pelves from denervated and innervated kidneys. There was no denervation-induced supersensitivity to noradrenaline or endothelin-1. Renal denervation did not increase pelvic connexin37, 40, 43 or 45 mRNA abundances. Gap junction blockade had no effect on spontaneous pelvic contractile activity. Conclusions The denervation-induced effect on pelvic pressure may be the consequence of the enhanced diuresis. The mechanisms underlying the denervation-induced effects on pelvic contraction frequency remain unknown. Our data rule out a major role for two important candidates, by showing that renal denervation neither induced supersensitivity to contractile agonists nor increased connexin mRNA abundance in the pelvic wall. |
| Databáze: | OpenAIRE |
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