Paternal heterochromatin formation in human embryos is H3K9/HP1 directed and primed by sperm-derived histone modifications
| Autor: | Godfried W. van der Heijden, Antoine H.F.M. Peters, Mareike Albert, Cindy Eleveld, Willy M. Baarends, Joop S.E. Laven, Esther B. Baart, Christine van de Werken, Miriam Teeuwssen |
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| Přispěvatelé: | Obstetrics & Gynecology, Developmental Biology |
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Male
Heterochromatin Chromosomal Proteins Non-Histone Zygote Inheritance Patterns General Physics and Astronomy Polycomb-Group Proteins Fertilization in Vitro Biology General Biochemistry Genetics and Molecular Biology Article Histones Mice Oogenesis Species Specificity Polycomb-group proteins Constitutive heterochromatin Animals Humans Epigenetics Spermatogenesis Genetics Multidisciplinary Embryonic cleavage Gene Expression Regulation Developmental General Chemistry Embryo Mammalian Spermatozoa Chromatin Histone Blastocyst Chromobox Protein Homolog 5 Fertilization biology.protein Oocytes Heterochromatin protein 1 Female |
| Zdroj: | Nature Communications Nature Communications, 5. Nature Publishing Group |
| ISSN: | 2041-1723 |
| Popis: | The different configurations of maternal and paternal chromatin, acquired during oogenesis and spermatogenesis, have to be rearranged after fertilization to form a functional embryonic genome. In the paternal genome, nucleosomal chromatin domains are re-established after the protamine-to-histone exchange. We investigated the formation of constitutive heterochromatin (cHC) in human preimplantation embryos. Our results show that histones carrying canonical cHC modifications are retained in cHC regions of sperm chromatin. These modified histones are transmitted to the oocyte and contribute to the formation of paternal embryonic cHC. Subsequently, the modifications are recognized by the H3K9/HP1 pathway maternal chromatin modifiers and propagated over the embryonic cleavage divisions. These results are in contrast to what has been described for mouse embryos, in which paternal cHC lacks canonical modifications and is initially established by Polycomb group proteins. Our results show intergenerational epigenetic inheritance of the cHC structure in human embryos. Following fertilization, the oocyte and sperm lose their distinct chromatin signature to form a functional embryonic genome. Here the authors find that, in human embryos, the paternal constitutive heterochromatin is inherited in the canonical configuration from the sperm and is propagated by the H3K9/HP1 pathway. |
| Databáze: | OpenAIRE |
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