Characterization of Stem-Like Cells Directly Isolated from Freshly Resected Laryngeal Squamous Cell Carcinoma Specimens
| Autor: | Ilknur Suer, Betul Yuceturk, Omer Faruk Karatas, Mehmet Yilmaz, Gülgün S. Güven, Mustafa Ozen, Harun Cansiz, Buge Oz |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Adult
Male Pathology medicine.medical_specialty Medicine (miscellaneous) Cell Separation CXCR4 Kruppel-Like Factor 4 SOX2 Antigens CD Cancer stem cell Tumor Cells Cultured medicine Humans AC133 Antigen Laryngeal Neoplasms Aged Glycoproteins Aged 80 and over biology CD44 Cancer General Medicine Middle Aged medicine.disease Head and neck squamous-cell carcinoma Hyaluronan Receptors KLF4 embryonic structures Carcinoma Squamous Cell Neoplastic Stem Cells Cancer research biology.protein Female sense organs biological phenomena cell phenomena and immunity Stem cell Peptides |
| Zdroj: | ResearcherID |
| ISSN: | 1574-888X |
| Popis: | Larynx cancer (LCa) is an aggressive malignancy, which is the second most common malignant neoplasm of head and neck squamous cell carcinoma. Its incidences have been reported to increase and therapeutic options mostly fail to give positive clinical response especially for the advanced LCa cases. In this study we aimed to isolate stem-like cells from freshly resected LCa tumor specimens and characterize them by quantitative real time PCR (qRT-PCR) for expression of cancer stem cell markers including SOX2, OCT4, KLF4, ABCG2, CXCR4 and CD44. Our results showed that CD133(high) cells directly isolated from freshly resected tumor specimens exhibit elevated levels of SOX2, OCT4 and KLF4, and have increased expression levels of ABCG2 and CXCR4, which were associated with resistance of tumors to regular chemotherapeutic reagents. In conclusion, this study offers a useful approach utilizing CD133 to isolate stem cells directly from fresh tissues, which gives the opportunity to develop novel therapeutic tools specifically targeting these cells through their further characterization. |
| Databáze: | OpenAIRE |
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