The adhesin related 30-kDa protein of Mycoplasma pneumoniae exhibits size and antigen variability
| Autor: | Gerlinde Layh-Schmitt, Ralf Himmelreich, Ute Leibfried |
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| Rok vydání: | 2006 |
| Předmět: |
Antigenicity
Mycoplasma pneumoniae Base Sequence biology Immunoblotting Molecular Sequence Data Mutant Filamentous haemagglutinin adhesin Cathepsin A Carboxypeptidases medicine.disease_cause Microbiology Carboxypeptidase Molecular Weight Bacterial adhesin Structure-Activity Relationship Antigen Mutation Protein A/G Genetics biology.protein medicine Adhesins Bacterial Molecular Biology |
| Zdroj: | FEMS Microbiology Letters. 152:101-108 |
| ISSN: | 0378-1097 |
| DOI: | 10.1111/j.1574-6968.1997.tb10415.x |
| Popis: | Mycoplasma pneumoniae is a pathogenic bacterium colonizing epithelial cells of the human respiratory tract. Using an erythrocyte binding assay we isolated a cytadsorption negative mutant designated M7 which has lost 12 of a total of 13 repetitive sequences of a proline rich C-terminal region of the adhesin related 30-kDa protein. The truncated adhesin related protein of 22 kDa showed reduced antigenicity compared to the corresponding wild-type protein. Moreover, the mutant M7 proved incapable of adhering to erythrocytes and to a human colon carcinoma cell line indicating that the repetitive C-terminal region of the 30-kDa protein is essential for effective cytadherence. The adhesin related 30-kDa protein as well as the truncated forms of the corresponding protein were accessible to carboxypeptidase Y which clearly shows surface exposure of the C-terminus of this protein. |
| Databáze: | OpenAIRE |
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