CD38 Expression in Neutrophils From Patients With Localized Aggressive Periodontitis
Autor: | Martha L. Warbington, Hidemi Kurihara, Tsuyoshi Fujita, Khalid H. Zawawi, Alpdogan Kantarci, Thomas E. Van Dyke, Hatice Hasturk |
---|---|
Rok vydání: | 2005 |
Předmět: |
Adult
Male medicine.medical_specialty Adolescent Neutrophils Blotting Western Stimulation CD38 Biology Article Basal (phylogenetics) chemistry.chemical_compound immune system diseases hemic and lymphatic diseases Internal medicine medicine Humans Aggressive periodontitis Periodontitis Chemotactic Factors Membrane Proteins hemic and immune systems Chemotaxis N-Formylmethionine leucyl-phenylalanine medicine.disease ADP-ribosyl Cyclase 1 N-Formylmethionine Leucyl-Phenylalanine Blot Chemotaxis Leukocyte Endocrinology chemistry Immunology Periodontics Female Densitometry |
Zdroj: | Journal of Periodontology. 76:1960-1965 |
ISSN: | 1943-3670 0022-3492 |
Popis: | Background: Localized aggressive periodontitis (LAgP) is associated with neutrophil dysfunction including decreased chemotaxis and reduced calcium entry. It has been suggested that CD38 is involved in chemotaxis. Little is known, however, about the relationship of CD38 and LAgP patients. In this study, we focused on the level of CD38 expression between LAgP and normal subjects and examined the involvement of CD38 in abnormal neutrophil chemotaxis of LAgP patients. Methods: Neutrophils from 12 normal subjects and 12 LAgP patients were isolated from peripheral venous blood. Membrane associated proteins were extracted from cells with or without N-formylmethionine leucyl-phenylalanine (fMLP) stimulation. CD38 expression was measured using Western blotting. Band density was measured using an imaging densitometer. Results: There was no statistical difference between normal subjects and LAgP patients in resting CD38 expression (basal level). However, the fMLP-stimulated neutrophils exhibited a significant decrease of CD38 expression in LAgP subjects compared to normal subjects. The decrease of CD38 was positively correlated with the defect in chemotactic migration to fMLP. Conclusion: These data suggest that the lower expression of CD38 in neutrophils may be related to altered neutrophil function in LAgP. |
Databáze: | OpenAIRE |
Externí odkaz: |