Human hepatic progenitor cells express vasoactive intestinal peptide receptor type 2 and receive nerve endings
| Autor: | Sara Vander Borght, David Cassiman, Jos van Pelt, Ilse Bockx, Bryon E. Petersen, Louis Libbrecht, Tania Roskams, Frederik Nevens, Nicoletta Sinelli, Rita Vos |
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| Rok vydání: | 2007 |
| Předmět: |
medicine.medical_specialty
Pathology Receptors Vasoactive Intestinal Polypeptide Type I Liver cytology Vasoactive intestinal peptide Biology Autonomic Nervous System Internal medicine Muscarinic acetylcholine receptor medicine Animals Humans Receptor Nerve Endings Hepatology Reverse Transcriptase Polymerase Chain Reaction Stem Cells Vasoactive intestinal peptide receptor Immunohistochemistry Rats Endocrinology Liver Receptors Vasoactive Intestinal Peptide Type II VIPR1 Free nerve ending Immunostaining |
| Zdroj: | Liver International. 27:323-328 |
| ISSN: | 1478-3231 1478-3223 |
| DOI: | 10.1111/j.1478-3231.2006.01427.x |
| Popis: | Background: We recently showed that human hepatic progenitor cells (HPCs) express muscarinic acetylcholine (Ach) receptor subtype 3 and that – following liver transplantation – HPC numbers are significantly reduced. To further elaborate on this, we examined whether HPC also express receptors for vasoactive intestinal peptide (VIP), which, besides Ach, also is an important parasympathetic neurotransmitter. VIP expressing nerves are known to be present in the liver. Methods: We performed immunohistochemistry for VIP receptor subtypes 1 and 2 (VIPR1 and 2), on sections of normal and diseased human liver (n=17), and double staining for VIPR2 and known HPC markers. We performed RT-PCR for VIPR1 and 2 on total RNA from purified rat HPC. To document the probability of direct interaction, we also performed double immunostaining for nerve markers and HPC markers on human liver sections. Results: VIPR2 immunostaining was clearly positive in HPC and reactive bile ductules on paraffin-embedded and frozen tissue sections. We could not demonstrate VIPR1 protein expression in the liver, with either of two VIPR1 antibodies tested. The presence of VIPR2 mRNA in HPC was confirmed by RT-PCR. Nerve endings were shown to abut on reactive bile ductules. Conclusion: We show here for the first time that HPC express VIPR2 and receive nerve endings. These features, and the fact that HPC numbers are influenced by the presence or absence of the autonomic innervation of the liver, suggest a direct interaction. |
| Databáze: | OpenAIRE |
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