Differential effects of silencing crustacean hyperglycemic hormone gene expression on the metabolic profiles of the muscle and hepatopancreas in the crayfish Procambarus clarkii
| Autor: | Shih Fu Tsai, Chi Ying Lee, Li Jane Shih, Yi Chun Tien, Wenfeng Li, Chien Hsun Lee, Jean Toullec, Kuo-Hsun Chiu |
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| Přispěvatelé: | National Changhua University of Education (NCUE), National Kaohsiung Marine University [Taïwan] (NKMU), Taoyuan Armed Forces General Hospital, Adaptation et Biologie des Invertébrés en Conditions Extrêmes (ABICE), Adaptation et diversité en milieu marin (AD2M), Station biologique de Roscoff [Roscoff] (SBR), Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Station biologique de Roscoff [Roscoff] (SBR), Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), National Changhua University of Education |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Metabolic Processes Glycogens Invertebrate Hormones Glycobiology lcsh:Medicine Hepatopancreas Gene Expression Astacoidea Biochemistry 0302 clinical medicine Hemolymph Gene expression Metabolites Medicine and Health Sciences Glycolysis lcsh:Science Multidisciplinary Organic Compounds Muscles Monosaccharides Crustaceans Eyestalk Chemistry Physical Sciences Metabolome Carbohydrate Metabolism Anatomy Research Article medicine.medical_specialty Arthropoda Carbohydrates Nerve Tissue Proteins Biology Carbohydrate metabolism Arthropod Proteins 03 medical and health sciences Internal medicine medicine Genetics Animals Gene Silencing RNA Double-Stranded Procambarus clarkii lcsh:R Organic Chemistry Chemical Compounds Organisms Biology and Life Sciences biology.organism_classification Invertebrates Crayfish Citric acid cycle 030104 developmental biology Endocrinology Metabolism Glucose Biological Tissue Gene Expression Regulation lcsh:Q Ganglia [SDE.BE]Environmental Sciences/Biodiversity and Ecology Energy Metabolism 030217 neurology & neurosurgery |
| Zdroj: | PLoS ONE PLoS ONE, 2017, 12 (2), pp.e0172557. ⟨10.1371/journal.pone.0172557.s004⟩ PLoS ONE, Public Library of Science, 2017, 12 (2), pp.e0172557. ⟨10.1371/journal.pone.0172557.s004⟩ PLoS ONE, Vol 12, Iss 2, p e0172557 (2017) |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0172557.s004⟩ |
| Popis: | International audience; In order to functionally characterize the metabolic roles of crustacean hyperglycemic hormone (CHH), gene expression of CHH in the crayfish (Procambarus clarkii) was knocked down by in vivo injection of CHH double-stranded RNA (dsRNA), followed by metabolomic analysis of 2 CHH target tissues (the muscle and hepatopancreas) using nuclear magnetic resonance spectroscopy. Compared to the levels in untreated and saline-injected (SAI) animals, levels of CHH transcript, but not those of molt-inhibiting hormone (a CHH-family peptide), in the eyestalk ganglia of CHH dsRNA-injected (DSI) animals were significantly decreased at 24, 48, and 72 hour post injection (hpi), with concomitant changes in levels of CHH peptide in the sinus gland (a neurohemal organ) and hemolymph. Green fluorescence protein (GFP) dsRNA failed to affect levels of CHH transcript in the eyestalk ganglia of GFP DSI animals. Number of metabolites whose levels were significantly changed by CHH dsRNA was 149 and 181 in the muscle and 24 and 12 in the hepatopancreas, at 24 and 48 hpi, respectively. Principal component analysis of these metabolites show that metabolic effects of silencing CHH gene expression were more pronounced in the muscle (with the cluster of CHH DSI group clearly being separated from that of SAI group at 24 hpi) than in the hepatopancreas. Moreover, pathway analysis of the metabolites closely related to carbohydrate and energy metabolism indicate that, for CHH DSI animals at 24 hpi, metabolic profile of the muscle was characterized by reduced synthesis of NAD+ and adenine ribonucleotides, diminished levels of ATP, lower rate of utilization of carbohydrates through glycolysis, and a partially rescued TCA cycle, whereas that of the hepatopancreas by unaffected levels of ATP, lower rate of utilization of carbohydrates, and increased levels of ketone bodies. The combined results of metabolic changes in response to silenced CHH gene expression reveal that metabolic functions of CHH on the muscle and hepatopancreas are more diverse than previously thought and are differential between the two tissues. |
| Databáze: | OpenAIRE |
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