| Autor: |
Isabella Stocker, Johannes Grillari, Jarod Rollins, Santina Snow, Clemens Heissenberger, Denis L. J. Lafontaine, Fabian Nagelreiter, Teresa L Krammer, Anton Shpylovyi, Aric N. Rogers, Markus Schosserer, Ludivine Wacheul |
| Jazyk: |
angličtina |
| Rok vydání: |
2020 |
| Předmět: |
|
| DOI: |
10.1101/2020.03.16.993469 |
| Popis: |
Our knowledge about the repertoire of ribosomal RNA modifications and the enzymes responsible for installing them is constantly expanding. Previously, we reported that NSUN-5 is responsible for depositing m5C at position C2381 on the 26S rRNA in Caenorhabditis elegans.Here, we show that NSUN-1 is writing the second known 26S rRNA m5C at position C2982. Depletion of nsun-1 or nsun-5 improved locomotion at midlife and resistance against heat stress, however, only soma-specific knockdown of nsun-1 extended lifespan. Moreover, soma-specific knockdown of nsun-1 reduced body size and impaired fecundity, suggesting non-cell-autonomous effects. While ribosome biogenesis and global protein synthesis were unaffected by nsun-1 depletion, translation of specific mRNAs was remodelled leading to reduced production of collagens, loss of structural integrity of the cuticle and impaired barrier function.We conclude that loss of a single enzyme required for rRNA methylation has profound and highly specific effects on organismal physiology. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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