Open chromatin profiling identifies AP1 as a transcriptional regulator in oesophageal adenocarcinoma
| Autor: | Britton, Edward, Rogerson, Connor, Mehta, Shaveta, Li, Yaoyong, Li, Xiaodun, OCCAMS Consortium, Fitzgerald, Rebecca C, Ang, Yeng S, Sharrocks, Andrew D |
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| Přispěvatelé: | Mehta, Shaveta [0000-0003-4990-3975], Fitzgerald, Rebecca C [0000-0002-3434-3568], Sharrocks, Andrew D [0000-0001-7395-9552], Apollo - University of Cambridge Repository |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
Transcriptional Activation
Esophageal Neoplasms Proto-Oncogene Proteins c-ets lcsh:QH426-470 Adenocarcinoma Chromatin Assembly and Disassembly Chromatin DNA-Binding Proteins Gene Expression Regulation Neoplastic Transcription Factor AP-1 lcsh:Genetics Cell Line Tumor Gene Knockdown Techniques Proto-Oncogene Proteins Humans Adenovirus E1A Proteins sense organs skin and connective tissue diseases Transcription Factors |
| Zdroj: | PLoS Genetics, Vol 13, Iss 8, p e1006879 (2017) Britton, E, Rogerson, C, Mehta, S, Li, Y, Li, X, OCCAMS consortium, Fitzgerald, R C, Ang, Y & Sharrocks, A 2017, ' Open chromatin profiling identifies AP1 as a transcriptional regulator in oesophageal adenocarcinoma ', PL o S Genetics, vol. 13, no. 8, e1006879 . https://doi.org/10.1371/journal.pgen.1006879 |
| ISSN: | 1553-7404 1553-7390 |
| DOI: | 10.1371/journal.pgen.1006879 |
| Popis: | Oesophageal adenocarcinoma (OAC) is one of the ten most prevalent forms of cancer and is showing a rapid increase in incidence and yet exhibits poor survival rates. Compared to many other common cancers, the molecular changes that occur in this disease are relatively poorly understood. However, genes encoding chromatin remodeling enzymes are frequently mutated in OAC. This is consistent with the emerging concept that cancer cells exhibit reprogramming of their chromatin environment which leads to subsequent changes in their transcriptional profile. Here, we have used ATAC-seq to interrogate the chromatin changes that occur in OAC using both cell lines and patient-derived material. We demonstrate that there are substantial changes in the regulatory chromatin environment in the cancer cells and using this data we have uncovered an important role for ETS and AP1 transcription factors in driving the changes in gene expression found in OAC cells. |
| Databáze: | OpenAIRE |
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