Phytochemical Profile and Anticholinesterase and Antimicrobial Activities of Supercritical versus Conventional Extracts of Satureja montana
| Autor: | Filipa V.M. Silva, Nuno R. Neng, Natália Gaspar, Joana Salta, António M. S. Palavra, Amélia P. Rauter, José M.F. Nogueira, Jorge Justino, Alice Martins, Clara Grosso, Delfina Mira, José S. Urieta |
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| Rok vydání: | 2009 |
| Předmět: |
food.ingredient
Satureja montana Extracto Bacillus Microbial Sensitivity Tests Chemical Fractionation HPLC-DAD Satureja Supercritical fluid extraction Protocatechuic acid chemistry.chemical_compound food Anti-Infective Agents Chlorogenic acid Oils Volatile Vanillic acid Plants Medicinal antimicrobial activity Chromatography biology Plant Extracts Anticholinesterase activity Chromatography Supercritical Fluid General Chemistry Plant Components Aerial Alzheimer's disease biology.organism_classification Supercritical fluid Phytochemical chemistry Planta medicinal Winter savory Cholinesterase Inhibitors General Agricultural and Biological Sciences Doença dos sistema nervoso Phytotherapy |
| Zdroj: | Repositório Científico de Acesso Aberto de Portugal Repositório Científico de Acesso Aberto de Portugal (RCAAP) instacron:RCAAP |
| ISSN: | 1520-5118 0021-8561 |
| DOI: | 10.1021/jf901786p |
| Popis: | Winter savory Satureja montana is a medicinal herb used in traditional gastronomy for seasoning meats and salads. This study reports a comparison between conventional (hydrodistillation, HID, and Soxhlet extraction, SE) and alternative (supercritical fluid extraction, SFE) extraction methods to assess the best option to obtain bioactive compounds. Two different types of extracts were tested, the volatile (SFE-90 bar, second separator vs HID) and the nonvolatile fractions (SFE-250 bar, first and second separator vs SE). The inhibitory activity over acetyl- and butyrylcholinesterase by S. montana extracts was assessed as a potential indicator for the control of Alzheimer's disease. The supercritical nonvolatile fractions, which showed the highest content of (+)-catechin, chlorogenic, vanillic, and protocatechuic acids, also inhibited selectively and significantly butyrylcholinesterase, whereas the nonvolatile conventional extract did not affect this enzyme. Microbial susceptibility tests revealed the great potential of S. montana volatile supercritical fluid extract for the growth control and inactivation of Bacillus subtilis and Bacillus cereus, showing some activity against Botrytis spp. and Pyricularia oryzae. Although some studies were carried out on S. montana, the phytochemical analysis together with the biological properties, namely, the anticholinesterase and antimicrobial activities of the plant nonvolatile and volatile supercritical fluid extracts, are described herein for the first time. |
| Databáze: | OpenAIRE |
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