AKTIVITAS ANTIBAKTERI PROTEIN KAPANG Xylaria psidii KT30 TERHADAP Escherichia coli DAN Bacillus subtilis [Antibacterial Activity of Protein Fungus Xylaria psidii KT30 on Escherichia coli and Bacillus subtilis]
| Autor: | A. Z. Mustopa, Kustiariyah Tarman, Aris Munandar, Tati Nurhayati |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
lcsh:TP368-456
biology Bacillus pumilus marine fungi Pseudomonas Xylaria psidii Bacillus subtilis biology.organism_classification medicine.disease_cause Microbiology antibacterial lcsh:Food processing and manufacture Sephadex protein purification Protein purification medicine Extracellular Antibacterial activity Escherichia coli |
| Zdroj: | Jurnal Teknologi dan Industri Pangan, Vol 25, Iss 2, Pp 146-151 (2014) |
| ISSN: | 2087-751X 1979-7788 |
| DOI: | 10.6066/jtip.2014.25.2.146 |
| Popis: | Previous research shows that extracellular protein of an algicolous fungus Xylaria psidii KT30 inhibited Bacillus pumilus, Listeria sp., Salmonella typhi, Staphylacoccus aureus, and Pseudomonas sp. with an average clear zone diameter of 7 mm. To enhance the potent antibacterial activity of extracellular protein from Xylaria psidii KT30, t he present research demonstrated fungal growth optimization and purification of its secreted extracellular protein. T he fungal growth optimization was performed following NaCl concentration and harvest day variations . The protein was p recipitat ed using ammonium sulphate at 60%-90% saturation range and was purifi ed through gel chromatography filtration using Sephadex G-50, eluted with 30% aq methanol . The active fraction possesing a ntibacterial activit y was then determined resulting supernatant, pellet, and protein fraction. The fungal was optimally obtained after 15 days cultivation using freshwater.The highest protein yield was 1.67% , resulted over 90% saturation. Fractions 11 and 12 were the most active against Escherichia coli dan Bacillus subtilis with clear zone diameter of 8 mm. Three bands of those fractions were detected through SDS-PAGE analysis, revealing molecular weights of 23.42, 20.09 , and 14.33 kDa. |
| Databáze: | OpenAIRE |
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