Lymphocyte populations during tuberculosis infection: V beta repertoires
| Autor: | T Cerdá Mota, M Pacheco Carracedo, J. Montes Santiago, Francisco Gambón-Deza |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Pathology
medicine.medical_specialty Lymphocytosis Pleural effusion Receptors Antigen T-Cell alpha-beta T cell Lymphocyte Immunology Peritonitis Tuberculous Biology Microbiology T-Lymphocyte Subsets medicine Humans Lymphocyte Count IL-2 receptor Gene Rearrangement beta-Chain T-Cell Antigen Receptor Tuberculosis Pulmonary Immunity Cellular Receptors Interleukin-2 Tuberculosis Pleural T lymphocyte medicine.disease Infectious Diseases medicine.anatomical_structure Peripheral blood lymphocyte Leukocyte Common Antigens Parasitology medicine.symptom CD8 Research Article |
| Zdroj: | Infection and Immunity. 63:1235-1240 |
| ISSN: | 1098-5522 0019-9567 |
| DOI: | 10.1128/iai.63.4.1235-1240.1995 |
| Popis: | The immune response to Mycobacterium tuberculosis is mediated by T lymphocytes. We studied the changes in lymphocyte populations occurring in peripheral blood, pleural fluid, and ascites during tuberculosis infection. For this purpose, we compared recent-onset patients (newly converted to positive Mantoux reactions) with previously diagnosed patients (individuals with organic lesions). Recent infection was associated with peripheral blood lymphocytosis involving T lymphocytes expressing either T-cell receptor alpha/beta or gamma/delta. Lymphocytosis involved both CD4 and CD8 cells. On the other hand, we detected no changes in the distribution of peripheral blood lymphocyte populations in previously diagnosed patients. No changes were found in the numbers of B lymphocytes or natural killer cells in either recently infected or previously diagnosed patients. The pleural effusion and ascitic fluid samples contained T lymphocytes expressing T-cell receptor alpha/beta, the majority of which were CD4+. These lymphocytes showed an inverted CD45RA-to-CD45RO ratio, and we found high-level expression of the interleukin-2 receptor (CD25) in some patients. The results are compatible with the existence of periods of cell activation in the pleural fluid (which are disclosed by the appearance of the CD25 antigen and the transition of CD45RA expression to CD45RO) together with nonactivation periods (loss of CD25 and persistence of CD45RO expression). We studied a fraction of the V beta repertoire in peripheral blood in both groups and the same fraction of the V beta repertoire in pleural fluid from patients with tuberculous pleuritis, demonstrating that, in recently infected subjects, lymphocytosis was produced by the increase in lymphocytes which expressed some specific V beta subfamilies that differed from one individual to another. In two of five patients studied, we found significant changes in the V beta repertoire between lymphocytes from peripheral blood and the pleural fluid samples. |
| Databáze: | OpenAIRE |
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