Expression of BRCA1 gene in ewe mammary epithelial cells during pregnancy: regulation by growth hormone and steroid hormones

Autor: I Gourdou, J.P. Peyrat, Jean Djiane, L Hornez, A Arnold, Karine Laud, L Belair
Přispěvatelé: Unité de biologie cellulaire et moléculaire, Institut National de la Recherche Agronomique (INRA), ProdInra, Migration
Rok vydání: 2001
Předmět:
Hydrocortisone
[SDV]Life Sciences [q-bio]
Endocrinology
Diabetes and Metabolism
Mammary gland
Genes
BRCA1
Sequence Homology
0302 clinical medicine
Endocrinology
Pregnancy
Cloning
Molecular
ComputingMilieux_MISCELLANEOUS
In Situ Hybridization
Progesterone
0303 health sciences
Estradiol
Reverse Transcriptase Polymerase Chain Reaction
General Medicine
3. Good health
[SDV] Life Sciences [q-bio]
medicine.anatomical_structure
030220 oncology & carcinogenesis
Female
DNA Probes
medicine.drug
Endocrine gland
medicine.medical_specialty
DNA
Complementary
medicine.drug_class
In situ hybridization
Biology
03 medical and health sciences
Mammary Glands
Animal
Internal medicine
Culture Techniques
medicine
Animals
Humans
Northern blot
RNA
Messenger
030304 developmental biology
Sheep
Base Sequence
Epithelial Cells
Blotting
Northern
Placental Lactogen
Prolactin
Hormones
Gene Expression Regulation
Estrogen
Growth Hormone
Hormone
Zdroj: European Journal of Endocrinology
European Journal of Endocrinology, BioScientifica, 2001, 145, pp.763-770
ISSN: 0804-4643
1479-683X
Popis: OBJECTIVE: Steroid hormones (estradiol and progesterone) in association with prolactin and growth hormone are involved in lobulo alveolar development of the mammary gland during pregnancy. We hypothesized that the BRCA1 gene may be induced by these different hormones. METHODS AND RESULTS: In this study, we have demonstrated by Northern blot and in situ hybridization, that the expression of ovine (o) BRCA1 mRNA in mammary epithelial cells increased dramatically during a short period in the second half of pregnancy (days 70 to 112) and decreased at the end of pregnancy. The increase in oBRCA1 mRNA expression is concomitant with rapid lobulo alveolar growth. Using an in vivo protocol to artificially induce mammary gland development, we demonstrated by the real-time RT-PCR method that growth hormone in association with estrogen, progesterone and hydrocortisone induces an increase of BRCA1 mRNA expression in the ewe mammary gland. Moreover, we showed that estradiol and progesterone induce oBRCA1 expression in primary cultures of ewe mammary gland. CONCLUSIONS: These results suggest that BRCA1 is a potential regulator of the effects of steroid hormones and growth hormone in the induction of mammary epithelial cell proliferation.
Databáze: OpenAIRE
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