Use of high-sensitivity digital ELISA improves the diagnostic performance of circulating brain-specific proteins for detection of traumatic brain injury during triage
| Autor: | Carolyn H Still, Megan L. Alder, Christine G. Smothers, Grant C. O’Connell, Shirley M. Moore, Allison R. Webel |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Adult Male medicine.medical_specialty Traumatic brain injury Neurofilament light Enzyme-Linked Immunosorbent Assay tau Proteins Gastroenterology Article 03 medical and health sciences 0302 clinical medicine Neurofilament Proteins Internal medicine Brain Injuries Traumatic Medicine Humans Aged Digital Technology Receiver operating characteristic medicine.diagnostic_test business.industry Brain Reproducibility of Results General Medicine Middle Aged medicine.disease Molecular diagnostics Triage 030104 developmental biology Neurology Blood biomarkers Immunoassay Female Neurology (clinical) business 030217 neurology & neurosurgery Biomarkers |
| Zdroj: | Neurol Res |
| Popis: | BACKGROUND: Historically, limited sensitivity associated with traditional immunoassay methods have prevented the use of brain-specific proteins as blood biomarkers of traumatic brain injury (TBI) during triage, as these proteins exhibit low concentrations in circulation. Digital ELISA is a newly-developed technique that is up to 1000 times more sensitive than conventional ELISA methods. The purpose of this study was to determine whether the use of digital ELISA over conventional ELISA methods improves the performance of brain-specific proteins as blood biomarkers of TBI during triage. METHODS: Blood was sampled from TBI patients (n=13) at emergency department admission, as well as from neurologically normal controls (n=72). Serum levels of two brain-specific proteins, neurofilament light chain (NfL) and Tau, were measured via digital ELISA. Estimated conventional ELISA measures were generated by adjusting values according to the lower limits of detection achievable with commercially available conventional ELISA assays, and receiver characteristic analysis (ROC) was used to compare the diagnostic performance of digital ELISA measures to estimated conventional ELISA measures in terms of their ability to discriminate between TBI patients and controls. RESULTS: Used in combination, Digital ELISA measures of NfL and Tau could discriminate between groups with 100% sensitivity and 91.7% specificity. Estimated conventional ELISA measures could only discriminate between groups with 7.7% sensitivity and 94.4% specificity. This difference in diagnostic performance was statistically significant according to a comparison of areas under ROC curves. CONCLUSIONS: The use of digital ELISA over conventional ELISA methods improves the diagnostic performance of circulating brain-specific proteins for detection of TBI during triage. |
| Databáze: | OpenAIRE |
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