Polycyclic Ketone Monooxygenase (PockeMO)
| Autor: | Marco W. Fraaije, Maximilian J. L. J. Fürst, Gonzalo de Gonzalo |
|---|---|
| Přispěvatelé: | Universidad de Sevilla. Departamento de Química orgánica, European Union (UE), European Union (UE). H2020, Biotechnology |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
Ketone
THERMOBIFIDA-FUSCA Sulfide Selective oxidations biocatalysis Chiral sulfoxides lcsh:Chemical technology 010402 general chemistry OXIDATION 01 natural sciences 4-HYDROXYACETOPHENONE MONOOXYGENASE Catalysis lcsh:Chemistry chemistry.chemical_compound REGENERATION Organic chemistry lcsh:TP1-1185 Physical and Theoretical Chemistry PHENYLACETONE-MONOOXYGENASE Alkyl General Environmental Science Phenylacetone monooxygenase Baeyer-Villiger monooxygenases chiral sulfoxides selective oxidations chemistry.chemical_classification 010405 organic chemistry Aryl CYCLOHEXANONE MONOOXYGENASE SITE Monooxygenase Toluene 0104 chemical sciences lcsh:QD1-999 chemistry Biocatalysis BAEYER-VILLIGER MONOOXYGENASES SULFIDES |
| Zdroj: | Catalysts idUS. Depósito de Investigación de la Universidad de Sevilla instname Catalysts; Volume 7; Issue 10; Pages: 288 Catalysts, 7(10):288, 1-10. MDPI AG Catalysts, Vol 7, Iss 10, p 288 (2017) |
| ISSN: | 2073-4344 |
| Popis: | recently discovered, moderately thermostable Baeyer-Villiger monooxygenase, polycyclic ketone monooxygenase (PockeMO), from Thermothelomyces thermophila has been employed as a biocatalyst in a set of asymmetric sulfoxidations. The enzyme was able to catalyze the oxidation of various alkyl aryl sulfides with good selectivities and moderate to high activities. The biocatalytic performance was able to be further increased by optimizing some reaction parameters, such as the addition of 10% v v− 1 of water miscible solvents or toluene, or by performing the conversion at a relatively high temperature (45 °C). PockeMO was found to display an optimum activity at sulfide concentrations of 50 mM, while it can also function at 200 mM. Taken together, the data show that PockeMO can be used as robust biocatalyst for the synthesis of optically active sulfoxides. European Union 635734 European Union H2020-LEIT BIO-2014-1 |
| Databáze: | OpenAIRE |
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