Transduction of the cellular src gene and 3' adjacent sequences in avian sarcoma virus PR2257

Autor: Karakoz I, Georges Calothy, Jean-Vianney Barnier, J. Svoboda, Philippe Dezélée, Jiří Nehyba, Bogdan A. Yatsula, Alla V. Rynditch, Josef Geryk
Přispěvatelé: CNRS UMR 146 - Institut Curie - Developmental Genetics of Melanocytes (CNRS), Centre National de la Recherche Scientifique (CNRS)
Jazyk: angličtina
Rok vydání: 1989
Předmět:
Genes
Viral
MESH: Oncogenes
[SDV.NEU.NB]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology
Retroviridae Proteins
MESH: Amino Acid Sequence
MESH: Base Sequence
Defective virus
MESH: Proviruses
Exon
Proviruses
Viral Envelope Proteins
Transduction
Genetic
MESH: Animals
MESH: Coturnix
Recombination
Genetic
Rous sarcoma virus
MESH: Genes
Viral
biology
[SDV.NEU.PC]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Psychology and behavior
MESH: Avian Sarcoma Viruses
Nucleic acid sequence
MESH: Chickens
Defective Viruses
[SDV.NEU.SC]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Cognitive Sciences
MESH: Defective Viruses
MESH: Transduction
Genetic
MESH: Neoplasms
Experimental
MESH: Recombination
Genetic
Avian lymphomatosis
Research Article
MESH: Oncogene Protein pp60(v-src)
Base pair
Immunology
Molecular Sequence Data
Proto-Oncogene Proteins pp60(c-src)
Coturnix
Microbiology
Avian sarcoma virus
MESH: Sequence Homology
Nucleic Acid
Oncogene Protein pp60(v-src)
Virology
Proto-Oncogene Proteins
Sequence Homology
Nucleic Acid
Proto-Oncogenes
Animals
Amino Acid Sequence
Gene
MESH: Molecular Sequence Data
Base Sequence
Neoplasms
Experimental
Oncogenes
MESH: Proto-Oncogene Proteins pp60(c-src)
MESH: Retroviridae Proteins
biology.organism_classification
Molecular biology
MESH: DNA
Viral
MESH: Proto-Oncogene Proteins
Avian Sarcoma Viruses
Insect Science
MESH: Proto-Oncogenes
MESH: Viral Envelope Proteins
DNA
Viral
Chickens
Zdroj: Journal of Virology
Journal of Virology, American Society for Microbiology, 1989, 63 (2), pp.481-92
Scopus-Elsevier
ISSN: 0022-538X
1098-5514
Popis: International audience; When injected into chickens, a transformation-defective mutant of the Prague C strain of Rous sarcoma virus induced tumors at low incidence and after a long latency. One such tumor released a replication-defective virus designated PR2257. We molecularly cloned and sequenced the proviral DNA from quail fibroblasts transformed by PR2257. Comparison of PR2257 sequence with that of Prague C, cellular src, and 3' adjacent cellular DNA showed that the spliced version of the c-src gene and about 950 base pairs (bp) of 3'-flanking cellular DNA were transduced into PR2257. This transduction eliminated nearly all replicative genes, since the gag gene splice donor site was linked to the splice acceptor site of the src gene and, on the 3' side, recombination occurred in the end of env gene. Insertion of two extra cytosines 23 bp before and 19 bp after the c-src stop codon resulted in an extension of the coding portion up to 587 amino acids, divergence of sequences after Pro-525 and replacement of Tyr-527 by a valine residue. In addition, it appears that the 5' and 3' untranslated regions of PR2257 result from multiple recombinations between exogenous and endogenous virus genomes. Limited digestion of p66src encoded by PR2257 with Staphylococcus aureus V8 protease yielded a V2 peptide (C-terminal moiety) with an apparent molecular mass of 31 kilodaltons, consistent with the 5.7-kilodalton increase expected from the DNA sequence. The structure of PR2257 suggests that the first step in the capture of c-src gene by avian lymphomatosis viruses is the trans splicing of the viral leader mRNA to exon 1 of c-src.
Databáze: OpenAIRE
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