Molecular profile of peripheral blood mononuclear cells from patients with rheumatoid arthritis
| Autor: | Marc Feldmann, Debra D. Pittman, Jonathan T Beech, Brian M. J. Foxwell, Jennifer A. Stover, Jeffrey L. Feldman, William L. Trepicchio, Christopher J Edwards, Kathleen M. Shields, Glenn Larsen, Fionula M. Brennan |
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| Jazyk: | angličtina |
| Rok vydání: | 2007 |
| Předmět: |
Adult
Candidate gene Transcription Genetic Inflammatory arthritis In Vitro Techniques Biology Peripheral blood mononuclear cell Fluorescence Arthritis Rheumatoid Gene expression Genetics medicine Cluster Analysis Humans Genetic Predisposition to Disease Molecular Biology Gene Genetics (clinical) Aged Oligonucleotide Array Sequence Analysis Nucleic Acid Hybridization Articles Middle Aged Gene signature medicine.disease Molecular medicine Gene Expression Regulation Case-Control Studies Immunology Leukocytes Mononuclear RNA Molecular Medicine Chromosomes Human Pair 6 Female Signal transduction |
| Zdroj: | Molecular medicine (Cambridge, Mass.). 13(1-2) |
| ISSN: | 1528-3658 1076-1551 |
| Popis: | Rheumatoid arthritis (RA) is a chronic inflammatory arthritis. Currently, diagnosis of RA may take several weeks, and factors used to predict a poor prognosis are not always reliable. Gene expression in RA may consist of a unique signature. Gene expression analysis has been applied to synovial tissue to define molecularly distinct forms of RA; however, expression analysis of tissue taken from a synovial joint is invasive and clinically impractical. Recent studies have demonstrated that unique gene expression changes can be identified in peripheral blood mononuclear cells (PBMCs) from patients with cancer, multiple sclerosis, and lupus. To identify RA disease-related genes, we performed a global gene expression analysis. RNA from PBMCs of 9 RA patients and 13 normal volunteers was analyzed on an oligonucleotide array. Compared with normal PBMCs, 330 transcripts were differentially expressed in RA. The differentially regulated genes belong to diverse functional classes and include genes involved in calcium binding, chaperones, cytokines, transcription, translation, signal transduction, extracellular matrix, integral to plasma membrane, integral to intracellular membrane, mitochondrial, ribosomal, structural, enzymes, and proteases. A k-nearest neighbor analysis identified 29 transcripts that were preferentially expressed in RA. Ten genes with increased expression in RA PBMCs compared with controls mapped to a RA susceptibility locus, 6p21.3. These results suggest that analysis of RA PBMCs at the molecular level may provide a set of candidate genes that could yield an easily accessible gene signature to aid in early diagnosis and treatment. |
| Databáze: | OpenAIRE |
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