Single beam optical tweezers setup with backscattered light detection for three-dimensional measurements on DNA and nanopores

Autor:	Katja Tönsing, Marcus Matthias Schafer, Wiebke Hachmann, Ina Seuffert, Dario Anselmetti, Andy Sischka, Christoph Kleimann
Rok vydání:	2008
Předmět:	Quantitative Biology::Biomolecules Materials science Infrared Rays business.industry Lasers Laser Bacteriophage lambda law.invention Optical axis Nanopore Optics Optical tweezers Optical microscope law DNA Viral Tweezers Perpendicular business Porosity Instrumentation Beam (structure)
Zdroj:	Review of Scientific Instruments. 79:063702
ISSN:	1089-7623 0034-6748
DOI:	10.1063/1.2938401
Popis:	We introduce a versatile and high precision three-dimensional optical tweezers setup with minimal optical interference to measure small forces and manipulate single molecules in the vicinity of a weak reflective surface. Our tweezers system integrates an inverted optical microscope with a single IR-laser beam that is spatially filtered in an appropriate way to allow force measurements in three dimensions with remarkably high precision when operated in backscattered light detection mode. The setup was tested by overstretching a lambda-DNA in x and z directions (perpendicular and along the optical axis), and by manipulating individual lambda-DNA molecules in the vicinity of a nanopore that allowed quantitative single molecule threading experiments with minimal optical interference.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7f12259d3d0fa213372c01e5b99f623b https://doi.org/10.1063/1.2938401 Zobrazit plný text záznamu