Activity of 5-formyl tetrahydrofolate cyclodehydrase and 5,10-methenyl tetrahydrofolate cyclohydrolase in primary brain tumors in children
| Autor: | Vasilis Evangelinos, Kanaris Panagopoulos, Fotini Tzortzatou-Stathopoulou, V. Mikraki, M. Moschovi, Athina Xaidara |
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| Rok vydání: | 1996 |
| Předmět: |
Ependymoma
medicine.medical_specialty Cyclohydrolase activity Biology Formate-Tetrahydrofolate Ligase Ligases Aminohydrolases Multienzyme Complexes Glioma Internal medicine medicine Humans Carbon-Nitrogen Ligases Purine metabolism Child chemistry.chemical_classification Medulloblastoma Methylenetetrahydrofolate Dehydrogenase (NADP) Brain Neoplasms Histocytochemistry Infant Hematology Metabolism medicine.disease Endocrinology Enzyme Oncology chemistry Child Preschool Pediatrics Perinatology and Child Health Immunohistochemistry |
| Zdroj: | Scopus-Elsevier |
| ISSN: | 0888-0018 |
| Popis: | The activity of the enzymes 5-formyl tetrahydrofolate cyclodehydrase and 5,10-methenyl tetrahydrofolate cyclohydrolase has been studied cytochemically in children's primary brain tumors. These enzymes play a significant role in purine biosynthesis. Thirty children, aged 1-12 years, were studied, 12 with medulloblastoma, 14 with glioma grade I-IV, and 4 with ependymoma. The activity of the enzymes was apparent as cytoplasmic granules that sometimes overlie the nucleus of the tumor cells. This coincidence showed that different types of brain tumors exhibit different degrees of enzymic activity, which in some cases correlated positively with the malignant potential of the tumor. Approximately one third of the cases were negative for any activity of these enzymes. The intensity of the staining of 5,10-methenyl tetrahydrofolate cyclohydrolase activity was actually higher than that of 5-formyl tetrahydrofolate cyctodehydrase. The clinical or prognostic significance of these findings remains to be clarified, but we believe that cylochemistry provides a sensitive technique for the detection, localization, and description of these enzymes in brain tumor cells. A clear understanding of the mode of action of these enzymes may contribute to devising novel therapeutic strategies. |
| Databáze: | OpenAIRE |
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