Determination of Biogenic Amines in Seawater Using Capillary Electrophoresis with Capacitively Coupled Contactless Conductivity Detection
| Autor: | Joselito P. Quirino, Bahruddin Saad, Elbaleeq Adam Gubartallah, Ahmad Makahleh |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Biogenic Amines
Capillary action Spermidine capillary electrophoresis Pharmaceutical Science 02 engineering and technology Electrolyte capacitively coupled contactless conductivity detector biogenic amines seawater Conductivity 01 natural sciences Article Analytical Chemistry lcsh:QD241-441 chemistry.chemical_compound Capillary electrophoresis lcsh:Organic chemistry Drug Discovery Seawater Physical and Theoretical Chemistry Detection limit Cadaverine Chromatography Chemistry 010401 analytical chemistry Organic Chemistry Electric Conductivity Electrophoresis Capillary 021001 nanoscience & nanotechnology 0104 chemical sciences Chemistry (miscellaneous) Putrescine Molecular Medicine Spermine 0210 nano-technology |
| Zdroj: | Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry Molecules; Volume 23; Issue 5; Pages: 1112 Molecules, Vol 23, Iss 5, p 1112 (2018) |
| ISSN: | 1420-3049 |
| Popis: | A rapid and green analytical method based on capillary electrophoresis with capacitively coupled contactless conductivity detection (C4D) for the determination of eight environmental pollutants, the biogenic amines (putrescine, cadaverine, spermidine, spermine, tyramine, 2-phenylamine, histamine and tryptamine), is described. The separation was achieved under normal polarity mode at 24 °C and 25 kV with a hydrodynamic injection (50 mbar for 5 s) and using a bare fused-silica capillary (95 cm length × 50 µm i.d.) (detection length of 10.5 cm from the outlet end of the capillary). The optimized background electrolyte consisted of 400 mM malic acid. C4D parameters were set at a fixed amplitude (50 V) and frequency (600 kHz). Under the optimum conditions, the method exhibited good linearity over the range of 1.0–100 µg mL−1 (R2 ≥ 0.981). The limits of detection based on signal to noise (S/N) ratios of 3 and 10 were ≤0.029 µg mL−1. The method was used for the determination of seawater samples that were spiked with biogenic amines. Good recoveries (77–93%) were found. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
| Nepřihlášeným uživatelům se plný text nezobrazuje | K zobrazení výsledku je třeba se přihlásit. |