Association of high molecular weight DNA fragmentation with apoptotic or non-apoptotic cell death induced by calcium ionophore
| Autor: | Akiro Okuda, Masaru Kubota, Kenshi Furusho, Ikuya Usami, Yoshihiro Wakazono, Akihiro Kataoka, Rikimaru Bessho, Yuichi Akiyama, Ying Wei Lin |
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| Rok vydání: | 1995 |
| Předmět: |
Cytoplasm
Necrosis Internucleosomal DNA fragmentation Biophysics chemistry.chemical_element Apoptosis Biology Calcium Biochemistry Leukemia Promyelocytic Acute Structural Biology Tumor Cells Cultured Genetics medicine Humans Magnesium Endonuclease Fragmentation (cell biology) Molecular Biology Calcimycin Cell Nucleus Terpenes Apoptotic DNA fragmentation food and beverages DNA Cell Biology Endonucleases Molecular biology High molecular weight DNA fragmentation Nucleosomes Molecular Weight chemistry Cell culture Thapsigargin DNA fragmentation medicine.symptom |
| Zdroj: | FEBS Letters. 364:264-267 |
| ISSN: | 0014-5793 |
| DOI: | 10.1016/0014-5793(95)00405-x |
| Popis: | Calcium ionophore (A23187)-induced high molecular weight (HMW) and internucleosomal DNA fragmentation were investigated in human leukemia cell lines. An apoptosis-sensitive cell line, HL-60, showed HMW, internucleosomal DNA fragmentation and morphological changes of apoptosis by A23187. MOLT-4, which is resistant to apoptosis, exhibited only HMW DNA fragmentation and died of necrosis under the same conditions. Autodigestion experiments suggested the endonucleolytic activity to cause HMW fragmentation in the cytoplasm of both cell lines. The activity was more dependent on Mg 2+ than Ca 2+ in HL-60, whereas it was Ca 2+ -dependent in MOLT-4. These results suggest that HMW DNA fragmentation is not specific to apoptosis. |
| Databáze: | OpenAIRE |
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