| Autor: |
Felix R. Jimenez-Rondan, Courtney H. Ruggiero, Kelley Lobean McKinley, Jin Koh, John F. Roberts, Eric W. Triplett, Robert J. Cousins |
| Rok vydání: |
2022 |
| Předmět: |
|
| Zdroj: |
American journal of physiology. Gastrointestinal and liver physiology. |
| ISSN: |
1522-1547 |
| Popis: |
Zinc, an essential micronutrient, has anti-inflammatory properties employing mechanisms that are unclear. Zip14 (Slc39a14) is a zinc transporter induced by proinflammatory stimuli and is highly expressed at the basolateral membrane of intestinal epithelial cells (IECs). Enterocyte-specific Zip14 ablation (Zip14ΔIEC) in mice was developed to study the role of this transporter in enterocytes. This gene deletion led to increased intestinal permeability, increased IL-6 and IFNγ expression, mild endotoxemia and intestinal dysbiosis. Tissues and intestinal epithelial cells were obtained from anesthetized mice. RNA sequencing was used for transcriptome profiling. These analyses revealed differential expression of specific intestinal proinflammatory and tight junction (TJ) genes. Binding of transcription factors, including NF-ĸβ, STAT3 and CDX2, to appropriate sites of promoters of these genes support the differential expression was shown with chromatin immunoprecipitation assays. Total HDAC and specifically HDAC3 activities were markedly reduced with Zip14 ablation. Intestinal organoids derived from ΔIEC mice display TJ and cytokine gene dysregulation compared to control mice. Differential expression of specific genes was reversed with zinc supplementation of the organoids. We conclude that zinc-dependent HDAC enzymes acquire zinc ions via Zip14-mediated transport and that intestinal integrity is controlled in part through epigenetic modifications. These studies potentially represent a new health-promoting role for this micronutrient. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
