Atomic Force microscopy reveals a role for endothelial cell ICAM-1 expression in bladder cancer cell adherence

Autor: Alain Duperray, Claude Verdier, Vinoth Sundar Rajan, Valérie M. Laurent
Přispěvatelé: Verdier, Claude, DYnamique des Fluides COmplexes et Morphogénèse [Grenoble] (DYFCOM), Laboratoire Interdisciplinaire de Physique [Saint Martin d’Hères] (LIPhy), Université Joseph Fourier - Grenoble 1 (UJF)-Centre National de la Recherche Scientifique (CNRS)-Université Joseph Fourier - Grenoble 1 (UJF)-Centre National de la Recherche Scientifique (CNRS), INSERM U823, équipe 8 (Immunologie Analytique des Pathologies Chroniques), Institut d'oncologie/développement Albert Bonniot de Grenoble (INSERM U823), Université Joseph Fourier - Grenoble 1 (UJF)-CHU Grenoble-EFS-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Joseph Fourier - Grenoble 1 (UJF)-CHU Grenoble-EFS-Institut National de la Santé et de la Recherche Médicale (INSERM), DYFCOM, Centre National de la Recherche Scientifique (CNRS)-Université Joseph Fourier - Grenoble 1 (UJF)-Centre National de la Recherche Scientifique (CNRS)-Université Joseph Fourier - Grenoble 1 (UJF), Institut National de la Santé et de la Recherche Médicale (INSERM)-EFS-CHU Grenoble-Université Joseph Fourier - Grenoble 1 (UJF)-Institut National de la Santé et de la Recherche Médicale (INSERM)-EFS-CHU Grenoble-Université Joseph Fourier - Grenoble 1 (UJF)
Jazyk: angličtina
Rok vydání: 2014
Předmět:
Ligands
Microscopy
Atomic Force
Biochemistry
Physical Chemistry
Forces
Epithelium
0302 clinical medicine
[SPI.MECA.BIOM] Engineering Sciences [physics]/Mechanics [physics.med-ph]/Biomechanics [physics.med-ph]
Molecular Cell Biology
Basic Cancer Research
Medicine and Health Sciences
CD43
Biomacromolecule-Ligand Interactions
Cancer
0303 health sciences
ICAM-1
Multidisciplinary
Leukosialin
biology
[PHYS.PHYS.PHYS-BIO-PH] Physics [physics]/Physics [physics]/Biological Physics [physics.bio-ph]
Chemistry
Physics
[SPI.MECA.BIOM]Engineering Sciences [physics]/Mechanics [physics.med-ph]/Biomechanics [physics.med-ph]
Intercellular Adhesion Molecule-1
Bladder Cancer
Invasiveness
Cell biology
Biomechanical Phenomena
Endothelial stem cell
Gene Expression Regulation
Neoplastic
medicine.anatomical_structure
Oncology
030220 oncology & carcinogenesis
Physical Sciences
Adhesion
Medicine
Anatomy
Cellular Types
AFM
Research Article
Endothelium
Science
[PHYS.PHYS.PHYS-BIO-PH]Physics [physics]/Physics [physics]/Biological Physics [physics.bio-ph]
Integrin
Biophysics
Receptor-ligand
MUC1
03 medical and health sciences
Cell Line
Tumor
medicine
Cell Adhesion
Humans
[PHYS.MECA.BIOM]Physics [physics]/Mechanics [physics]/Biomechanics [physics.med-ph]
Cell adhesion
030304 developmental biology
Intermolecular Forces
Mucin-1
[PHYS.MECA.BIOM] Physics [physics]/Mechanics [physics]/Biomechanics [physics.med-ph]
Biology and Life Sciences
Endothelial Cells
Cancers and Neoplasms
Epithelial Cells
Cell Biology
medicine.disease
Genitourinary Tract Tumors
Biological Tissue
Urinary Bladder Neoplasms
Chemical Properties
Cell culture
Cancer cell
biology.protein
Zdroj: PLoS ONE
PLoS ONE, 2014, 9 (5), pp.e98034
PLoS ONE, Public Library of Science, 2014, 9 (5), pp.e98034
PLoS ONE, Vol 9, Iss 5, p e98034 (2014)
ISSN: 1932-6203
Popis: International audience; Cancer metastasis is a complex process involving cell-cell interactions mediated by cell adhesive molecules. In this study we determine the adhesion strength between an endothelial cell monolayer and tumor cells of different metastatic potentials using Atomic Force Microscopy. We show that the rupture forces of receptor-ligand bonds increase with retraction speed and range between 20 and 70 pN. It is shown that the most invasive cell lines (T24, J82) form the strongest bonds with endothelial cells. Using ICAM-1 coated substrates and a monoclonal antibody specific for ICAM-1, we demonstrate that ICAM-1 serves as a key receptor on endothelial cells and that its interactions with ligands expressed by tumor cells are correlated with the rupture forces obtained with the most invasive cancer cells (T24, J82). For the less invasive cancer cells (RT112), endothelial ICAM-1 does not seem to play any role in the adhesion process. Moreover, a detailed analysis of the distribution of rupture forces suggests that ICAM-1 interacts preferentially with one ligand on T24 cancer cells and with two ligands on J82 cancer cells. Possible counter receptors for these interactions are CD43 and MUC1, two known ligands for ICAM-1 which are expressed by these cancer cells.
Databáze: OpenAIRE
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